Preprint Article Version 1 This version is not peer-reviewed

DNA Methylation Silencing of microRNA Gene Methylater in the Precancerous Background Mucosa with and without Gastric Cancer: Analysis of Effects of Helicobacter pylori Eradication and Long-Term Aspirin Use

Version 1 : Received: 6 February 2019 / Approved: 7 February 2019 / Online: 7 February 2019 (11:30:17 CET)

How to cite: Watari, J.; Ito, C.; Shimoda, T.; Tomita, T.; Oshima, T.; Fukui, H.; Das, K.; Miwa, H. DNA Methylation Silencing of microRNA Gene Methylater in the Precancerous Background Mucosa with and without Gastric Cancer: Analysis of Effects of Helicobacter pylori Eradication and Long-Term Aspirin Use. Preprints 2019, 2019020070 (doi: 10.20944/preprints201902.0070.v1). Watari, J.; Ito, C.; Shimoda, T.; Tomita, T.; Oshima, T.; Fukui, H.; Das, K.; Miwa, H. DNA Methylation Silencing of microRNA Gene Methylater in the Precancerous Background Mucosa with and without Gastric Cancer: Analysis of Effects of Helicobacter pylori Eradication and Long-Term Aspirin Use. Preprints 2019, 2019020070 (doi: 10.20944/preprints201902.0070.v1).

Abstract

The risk of gastric cancer (GC) declines after Helicobacter pylori (H. pylori) eradication and long-term aspirin use. We evaluated the effects of H. pylori eradication (Cohort 1) and aspirin use (Cohort 2) on the methylation of microRNAs (miRNAs) such as miR-34c, miR-124a-3, miR-129-2, and miR-137 in the gastric mucosa with and without GC, i.e., atrophic mucosa (AM) and intestinal metaplasia (IM). DNA was isolated from AM and IM separately using laser caption microdissection. In Cohort 1, H. pylori eradication was associated with a significant reduction of miR-124a-3 methylation only in AM, but not in IM. miR-129-2 methylation in AM may be a surrogate marker of GC in H. pylori-infected patients. In Cohort 2, aspirin did not reverse miRNA methylation in either AM or IM irrespective of H. pylori infection. miR-129-2 methylation in AM was an independent predictive marker of GC in H. pylori-infected but not -eradicated patients. These results indicate that H. pylori eradication and aspirin use were less effective in improving methylation in IM compared with AM; thus, these interventions are recommended at an early stage prior to the development of IM to prevent GC development.

Subject Areas

Helicobacter pylori, aspirin, atrophic gastritis, intestinal metaplasia, microRNA, methylation

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