Preprint Article Version 1 This version is not peer-reviewed

Transcriptional profile of Aedes aegypti Leucine-Rich Repeat Proteins in response to Zika and Chikungunya viruses

Version 1 : Received: 30 December 2018 / Approved: 3 January 2019 / Online: 3 January 2019 (12:12:05 CET)

A peer-reviewed article of this Preprint also exists.

Zhao, L.; Alto, B.W.; Shin, D. Transcriptional Profile of Aedes aegypti Leucine-Rich Repeat Proteins in Response to Zika and Chikungunya Viruses. Int. J. Mol. Sci. 2019, 20, 615. Zhao, L.; Alto, B.W.; Shin, D. Transcriptional Profile of Aedes aegypti Leucine-Rich Repeat Proteins in Response to Zika and Chikungunya Viruses. Int. J. Mol. Sci. 2019, 20, 615.

Journal reference: Int. J. Mol. Sci. 2019, 20, 615
DOI: 10.3390/ijms20030615

Abstract

Aedes aegypti (L.) is the primary vector of chikungunya, dengue, yellow fever and Zika viruses. The leucine-rich repeats (LRR)-containing domain is evolutionarily conserved in many proteins associated with innate immunity in invertebrates and vertebrates, as well as plants. We focused on the AaeLRIM1 and AaeAPL1 gene expressions in response to Zika virus (ZIKV) and Chikungunya virus (CHIKV) infection using a time course study, as well as the developmental expressions in the eggs, larvae, pupae, and adults. RNA-seq analysis data provided 60 leucine-rich repeat related transcriptions in Ae. aegypti in response to Zika virus (Accession number: GSE118858, https://www.ncbi.nlm.nih.gov/gds/?term=GSE118858). RNA-seq analysis data showed that AaeLRIM1 (AAEL012086-RA) and AaeAPL1 (AAEL009520-RA) were significantly upregulated 2.5 and 3-fold during infection by ZIKV 7-days post infection (dpi) of an Ae. aegypti Key West strain compared to an Orlando strain. The qPCR data showed that LRR-containing proteins AaeLRIM1, AaeAPL1 and five paralogues were expressed 100-fold lower than other nuclear genes, such as defensin, during all developmental stages examined. Together, these data provide insights into transcription profiles of LRR proteins of Ae. aegypti during its development and in response to infection with emergent arboviruses.

Subject Areas

Aedes aegypti, Leucine-Rich Repeat Proteins, Zika virus, Chikungunya virus, immune responses, gene expression

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