Preprint Article Version 1 This version is not peer-reviewed

Tracking the Healing Process of Experimental Liver Injuries Treated with Different Sealants and Adhesive Biomaterials: An Evaluation of Matrix Metalloproteinase

Version 1 : Received: 13 December 2018 / Approved: 17 December 2018 / Online: 17 December 2018 (10:52:27 CET)

How to cite: Lloris-Carsí, J.M.; Barrios-Pitarque, C.; Lloris-Cejalvo, J.M.; Gil-Santos, L.; Cejalvo-Lapeña, D. Tracking the Healing Process of Experimental Liver Injuries Treated with Different Sealants and Adhesive Biomaterials: An Evaluation of Matrix Metalloproteinase. Preprints 2018, 2018120192 (doi: 10.20944/preprints201812.0192.v1). Lloris-Carsí, J.M.; Barrios-Pitarque, C.; Lloris-Cejalvo, J.M.; Gil-Santos, L.; Cejalvo-Lapeña, D. Tracking the Healing Process of Experimental Liver Injuries Treated with Different Sealants and Adhesive Biomaterials: An Evaluation of Matrix Metalloproteinase. Preprints 2018, 2018120192 (doi: 10.20944/preprints201812.0192.v1).

Abstract

This study examines the matrix metalloproteinase (MMP) activity in the healing of liver injuries treated with biological adhesives Tachosil® and GelitaSpon® and the elastic cyanoacrylate Adhflex®. Hepatic lesions were induced in male rats using a Stiefel biopsy punch. Healing was assessed 2, 6, and 18 days after injury by quantifying tissue levels of MMP1, 2, 8, 9, and 13. Histopathological repair was evaluated using hematoxylin-eosin, Masson’s trichrome, and Periodic Acid Schiff (PAS) staining and immunohistochemical markers CD31 and CD68. The sealants contributed to complete healing. Histopathology and MMP findings indicate that Adhflex® has slower degradation and a strong inflammatory reaction at the onset of healing. Early on, all MMPs showed higher levels in Adhflex® and Tachosil®-treated animals, and MMP2 and MMP9 expressions were significantly higher in the Adhflex-treated group at 18 days post-injury (T3). The Adhflex® group had significantly higher MMP8 and MPP13 levels than other treated groups and showed a sustained overexpression of all MMPs, even in the latest healing stages. Notably, the overexpression did not negatively influence the histological healing process. All hepatic trauma injuries should be treated as emergencies, and any easy-to-use and rapid sealant like Adhflex® could be considered as an option for treating liver trauma.

Subject Areas

hepatic injury; sealants; metalloproteinases; inflammatory response; wound healing

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