Futai, N.; Tamura, M.; Ogawa, T.; Tanaka, M. Microfluidic Long-Term Gradient Generator with Axon Separation Prototyped by 185 nm Diffused Light Photolithography of SU-8 Photoresist. Micromachines2019, 10, 9.
Futai, N.; Tamura, M.; Ogawa, T.; Tanaka, M. Microfluidic Long-Term Gradient Generator with Axon Separation Prototyped by 185 nm Diffused Light Photolithography of SU-8 Photoresist. Micromachines 2019, 10, 9.
We have developed a cast microfluidic chip for concentration gradient generation that contains a thin (~5 μm^2 crosssectional area) microchannel. Durable 2 μm-high microchannel mold features with a smooth bell-shaped sidewall were fabricated by exposing SU-8 photoresist to diffused 185 nm UV light emitted by a low-cost ozone lamp from the backside of the substrate to ensure sufficient crosslinking of small regions of the SU-8 photoresist. An H-shaped microfluidic configuration was used, in which the thin channel was able to maintain constant diffusion fronts beyond purely static diffusion confirmed with experiment. We also demonstrated the long-term effects of a gradient of nerve growth factor on axon elongation by primary neuronal cells cultured in the microfluidic channel.
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