Preprint Article Version 1 This version is not peer-reviewed

Inonotus Obliquus extracts as an inhibitor of α-MSH-induced melanogenesis in B16F10 mouse melanoma cells

Version 1 : Received: 8 November 2018 / Approved: 9 November 2018 / Online: 9 November 2018 (13:36:36 UTC)

How to cite: Lee, E.; Cha, H.J. Inonotus Obliquus extracts as an inhibitor of α-MSH-induced melanogenesis in B16F10 mouse melanoma cells. Preprints 2018, 2018110247 (doi: 10.20944/preprints201811.0247.v1). Lee, E.; Cha, H.J. Inonotus Obliquus extracts as an inhibitor of α-MSH-induced melanogenesis in B16F10 mouse melanoma cells. Preprints 2018, 2018110247 (doi: 10.20944/preprints201811.0247.v1).

Abstract

Melanogenesis is a biosynthetic pathway for producing of the pigment melanin in human skin. Tyrosinase, a key enzyme, catalyzing is the first step in melanogenesis and the downregulation of the tyrosinase enzyme activity is the most reported method for anti-melanogenesis. According to the hyperpigmentation as an important issue in cosmetic industry, there is a big demand for melanogenesis inhibitors. In the present study, we identified the anti-melanogenic effect of Inonotus Obliquus in α-MSH-induced B16F10 mouse melanoma cells as a new inhibitor. Comparing with control and Inonotus Obliquus extracts treated B16F10, we identified melanin contents, tyrosinase activity, tyrosinase mRNA and protein expression, MITF activity using a constructed plasmid. As shown in these results, we demonstrated that Inonotus Obliquus extracts down-regulated melanin synthesis using down-regulating activity and expression of tyrosinase which is key enzyme to produce melanin. In addition, we revealed expression of tyrosinase is regulated by MITF through repressing MITF transcriptional activity. Inonotus Obliquus extracts has potential to repress melanogenesis and decreased of hyperpigmentation and to use as cosmetic ingredient.

Subject Areas

Inonotus Obliquus, Melanogenesis, B16F10, Tyrosinase, MITF

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