Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Primary Tree Shrew Dermis Fibroblasts Support Lytic Replication of Human Cytomegalovirus

Shu-Wei Dong
,
Ling-Shuai Jiao
ORCID logo ,
Ming Yang
,
Ying-Liang Duan
,
Yi-Bo Chen
,
Fei Zhao
,
A-Mei Zhang
,
Li Liu
,
Min-Hua Luo
* ,
Xue-Shan Xia
*
Version 1 : Received: 2 November 2018 / Approved: 5 November 2018 / Online: 5 November 2018 (09:43:16 CET)

How to cite: Dong, S.; Jiao, L.; Yang, M.; Duan, Y.; Chen, Y.; Zhao, F.; Zhang, A.; Liu, L.; Luo, M.; Xia, X. Primary Tree Shrew Dermis Fibroblasts Support Lytic Replication of Human Cytomegalovirus. Preprints 2018, 2018110095. https://doi.org/10.20944/preprints201811.0095.v1 Dong, S.; Jiao, L.; Yang, M.; Duan, Y.; Chen, Y.; Zhao, F.; Zhang, A.; Liu, L.; Luo, M.; Xia, X. Primary Tree Shrew Dermis Fibroblasts Support Lytic Replication of Human Cytomegalovirus. Preprints 2018, 2018110095. https://doi.org/10.20944/preprints201811.0095.v1

Abstract

As a universal pathogen leading to neonatal defects and transplant failure, Human cytomegalovirus (HCMV) has strict species specificity that the inability to using this virus in animals has hampered its pathogenesis study. However, the mechanism of cross-species barrier remains elusive that no non-human cell model has been established to fill this knowledge gap. We observed that primary dermis fibroblasts (TSDF) isolated from the Chinese tree shrew (Tupaia belangeri chinensis), a small laboratory animal with close affinity to primates, were permissive to HCMV replication. In TSDF infected with GFP-expressing HCMV, the green fluorescence and cytopathic effect were observed and the expression of 3 kinetic genes and replication of viral genome were detected. The cell-free viruses produced in TSDF reached 103 pfu/mL at 96 hpi, which were 10-fold lower than in primary human foreskin fibroblasts. Our results demonstrated that TSDF supported low level of lytic replication of HCMV. The TSDF model provides a useful platform for the mechanism study of species barrier of HCMV.

Keywords

Human cytomegalovirus; primary tree shrew dermis fibroblasts; cross-species infection; lytic replication.

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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