Preprint Article Version 1 This version is not peer-reviewed

Three Dimensional Coaxial Multi-Nozzle Device for High-Rate Microsphere Generation

Version 1 : Received: 2 November 2018 / Approved: 5 November 2018 / Online: 5 November 2018 (07:54:22 CET)

How to cite: Jaligama, S.; Kameoka, J. Three Dimensional Coaxial Multi-Nozzle Device for High-Rate Microsphere Generation. Preprints 2018, 2018110088 (doi: 10.20944/preprints201811.0088.v1). Jaligama, S.; Kameoka, J. Three Dimensional Coaxial Multi-Nozzle Device for High-Rate Microsphere Generation. Preprints 2018, 2018110088 (doi: 10.20944/preprints201811.0088.v1).

Abstract

Tissue engineering is an emerging field of research due to its growing impact on the regeneration of tissue injured from damage and organ failure. Small hydrogel microspheres containing regenerative cells and stimulators can be used as building blocks to regenerate tissue by injecting them into damaged areas; a microneedle is used to minimize the incision and any associated infections. To address the need for production of a large number of microspheres for use in tissue engineering, we developed a 3D coaxial multi-nozzle flow-focusing device fabricated via a simple 3D-printing method that demonstrates a high rate of microsphere production per nozzle. This device has six coaxial microscale nozzles that produce hydrogel microspheres. Two individual parts—the inlet and nozzle—can be made separately by a 3D printer and bonded together using uncured photo-curable resin as glue. The dimensions of the microspheres are between 100 μm and 1200 μm. They are produced by adjusting the flow rate ratio between the dispersed and continuous media. A flow rate ratio of 180 demonstrated the highest microsphere production rate of 2.12e+5 microspheres per sec (0.25 mL/min). This microsphere production rate per nozzle is four times higher than that of currently available devices.

Subject Areas

3D coaxial; multi-nozzle; flow-focusing; high- rate; microsphere; tissue engineering; stereolithography

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.