Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

NQO1 is Required for Beta-lapachone-mediated Downregulation of Breast Cancer Stem Cell Activity

Version 1 : Received: 29 October 2018 / Approved: 30 October 2018 / Online: 30 October 2018 (09:14:35 CET)

How to cite: Kim, D.W.; Cho, J. NQO1 is Required for Beta-lapachone-mediated Downregulation of Breast Cancer Stem Cell Activity. Preprints 2018, 2018100719. https://doi.org/10.20944/preprints201810.0719.v1 Kim, D.W.; Cho, J. NQO1 is Required for Beta-lapachone-mediated Downregulation of Breast Cancer Stem Cell Activity. Preprints 2018, 2018100719. https://doi.org/10.20944/preprints201810.0719.v1

Abstract

Background: Cancer stem cells (CSCs) exhibit self-renewal activity and give rise to other cell types in tumors. Due to the infinite proliferative potential of CSCs, drugs targeting these cells are necessary to completely inhibit cancer development. beta-lapachone (bL) has been widely used to treat cancer development, but its effect on cancer stem cells remain elusive. Thus, we investigated the effect of bL on mammosphere formation using breast cancer stem cell (BCSC) marker positive cells, MDA-MB-231. Methods: MDA-MB-231 Cells, which is negative for NQO1 expression, was constructed to stably express NQO1(NQO1 stable cells) to see the effect of bL. The effect of bL on cells were evaluated by wound healing and Transwell cell culture chambers, and ALDEFLUOR assay. Results: Here, we show that bL inhibited the proliferative ability of mammosphere derived from BCSC marker-positive cells, MDA-MB-231, in an NQO1-dependent manner. bL treatment efficiently downregulated expression level of BCSC markers CD44, ALDH1A1, and DLGAP5 that recently identified as a stem cell proliferation marker in both cultured cells and mammosphered cells. Moreover, bL efficiently downregulates cell proliferation and migration activities. Conclusions: These results strongly suggest that bL could be a therapeutic agent targeting breast cancer stem cells with proper NQO1 expression.

Keywords

beta-lapachone; Breast cancer stem cell; DLGAP5; Mammosphere; NQO1

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.