Preprint Article Version 1 This version is not peer-reviewed

Impedance Sensing Platform for Detection of the Food Pathogen Listeria Monocytogenes

Version 1 : Received: 25 October 2018 / Approved: 25 October 2018 / Online: 25 October 2018 (16:09:34 CEST)

A peer-reviewed article of this Preprint also exists.

Chiriacò, M.S.; Parlangeli, I.; Sirsi, F.; Poltronieri, P.; Primiceri, E. Impedance Sensing Platform for Detection of the Food Pathogen Listeria monocytogenes. Electronics 2018, 7, 347. Chiriacò, M.S.; Parlangeli, I.; Sirsi, F.; Poltronieri, P.; Primiceri, E. Impedance Sensing Platform for Detection of the Food Pathogen Listeria monocytogenes. Electronics 2018, 7, 347.

Journal reference: Electronics 2018, 7, 347
DOI: 10.3390/electronics7120347

Abstract

A great improvement in food safety and quality controls worldwide has been achieved through the development of biosensing platforms. Foodborne pathogens continue to cause serious outbreaks due to the ingestion of contaminated food. The development of new, sensitive, portable, high-throughput, and automated platforms is a primary objective to allow detection of pathogens and their toxins in foods. Listeria monocytogenes is one common foodborne pathogen. Major outbreaks of listeriosis have been caused by a variety of foods, including milk, soft cheeses, meat, fermented sausages, poultry, seafood and vegetable products. Due to its high sensitivity and easy setup, electrochemical impedance spectroscopy (EIS) has been extensively applied for biosensor fabrication and in particular in the field of microbiology as a mean to detect and quantify foodborne bacteria. Here we describe a miniaturized, portable EIS platform consisting of a microfluidic device with EIS sensors for the detection of L. monocytogenes in milk samples, connected to a portable impedance analyzer for on-field application in clinical and food diagnostics but also for biosecurity purposes. To achieve this goal microelectrodes were functionalized with antibodies specific for L. monocytogenes. The binding and detection of L. monocytogenes was achieved in the range 2.2 x 103 cfu/ml to 1 x 102 with a Limit of Detection (LoD) of 5.5 cfu/ml.

Subject Areas

food pathogens; Electrochemical impedance spectroscopy; surface activation; gold; microelectrodes

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