Caenorhabditis Elegans and Tenebrio Molitor - New Tools to Investigate Malassezia Species

Ana Paula Rabelo Silva; Aline Dias Valerio; Roberta Oliveira Viana; Ana Carolina dos Santos Ricoy; Susana Johann; Viviane de Souza Alves

doi:10.20944/preprints201810.0001.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 30 September 2018 / Approved: 1 October 2018 / Online: 1 October 2018 (10:12:54 CEST)

Malassezia species are part of human commensal microbiota and is also related to diseases. Little is known about the interaction of these microorganisms with their host. Here we established two standard culture conditions for Malassezia spp. to perform infection assays using C. elegans and T. molitor. Invertebrate hosts infected by Malassezia spp. cultured in M9M resulted in higher death rate on survival assays when compared to yeasts cultured in standard Dixon medium indicating that M9M cultured Malassezia species have increased virulence. The culture and infection conditions established in this work, using invertebrate alternative models, are valuable tools to understand Malassezia-host interaction.

Malassezia, Caenorhabditis elegans, Tenebrio molitor

Biology and Life Sciences, Immunology and Microbiology

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Caenorhabditis Elegans and Tenebrio Molitor - New Tools to Investigate Malassezia Species

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