Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Caenorhabditis Elegans and Tenebrio Molitor - New Tools to Investigate Malassezia Species

Version 1 : Received: 30 September 2018 / Approved: 1 October 2018 / Online: 1 October 2018 (10:12:54 CEST)

How to cite: Silva, A.P.R.; Valerio, A.D.; Viana, R.O.; Ricoy, A.C.D.S.; Johann, S.; Alves, V.D.S. Caenorhabditis Elegans and Tenebrio Molitor - New Tools to Investigate Malassezia Species. Preprints 2018, 2018100001. https://doi.org/10.20944/preprints201810.0001.v1 Silva, A.P.R.; Valerio, A.D.; Viana, R.O.; Ricoy, A.C.D.S.; Johann, S.; Alves, V.D.S. Caenorhabditis Elegans and Tenebrio Molitor - New Tools to Investigate Malassezia Species. Preprints 2018, 2018100001. https://doi.org/10.20944/preprints201810.0001.v1

Abstract

Malassezia species are part of human commensal microbiota and is also related to diseases. Little is known about the interaction of these microorganisms with their host. Here we established two standard culture conditions for Malassezia spp. to perform infection assays using C. elegans and T. molitor. Invertebrate hosts infected by Malassezia spp. cultured in M9M resulted in higher death rate on survival assays when compared to yeasts cultured in standard Dixon medium indicating that M9M cultured Malassezia species have increased virulence. The culture and infection conditions established in this work, using invertebrate alternative models, are valuable tools to understand Malassezia-host interaction.

Keywords

Malassezia, Caenorhabditis elegans, Tenebrio molitor

Subject

Biology and Life Sciences, Immunology and Microbiology

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