Version 1
: Received: 17 September 2018 / Approved: 17 September 2018 / Online: 17 September 2018 (15:05:44 CEST)
How to cite:
Hossain, M.; Brennan, M.; Mason, S.; Guo, X.; Zeng, X.-A.; Brennan, C. Blackcurrant Incorporated Biofunctional Cookie Digesta Shows Chemical and Cellular Biological Activity Against Human Liver Cancer Cells, HepG2. Preprints2018, 2018090319. https://doi.org/10.20944/preprints201809.0319.v1
Hossain, M.; Brennan, M.; Mason, S.; Guo, X.; Zeng, X.-A.; Brennan, C. Blackcurrant Incorporated Biofunctional Cookie Digesta Shows Chemical and Cellular Biological Activity Against Human Liver Cancer Cells, HepG2. Preprints 2018, 2018090319. https://doi.org/10.20944/preprints201809.0319.v1
Hossain, M.; Brennan, M.; Mason, S.; Guo, X.; Zeng, X.-A.; Brennan, C. Blackcurrant Incorporated Biofunctional Cookie Digesta Shows Chemical and Cellular Biological Activity Against Human Liver Cancer Cells, HepG2. Preprints2018, 2018090319. https://doi.org/10.20944/preprints201809.0319.v1
APA Style
Hossain, M., Brennan, M., Mason, S., Guo, X., Zeng, X. A., & Brennan, C. (2018). Blackcurrant Incorporated Biofunctional Cookie Digesta Shows Chemical and Cellular Biological Activity Against Human Liver Cancer Cells, HepG2. Preprints. https://doi.org/10.20944/preprints201809.0319.v1
Chicago/Turabian Style
Hossain, M., Xin-An Zeng and Charles Brennan. 2018 "Blackcurrant Incorporated Biofunctional Cookie Digesta Shows Chemical and Cellular Biological Activity Against Human Liver Cancer Cells, HepG2" Preprints. https://doi.org/10.20944/preprints201809.0319.v1
Abstract
The improved understanding of the underlying mechanisms of oxidative damage and/or chronic diseases is of high priority in dietary research. Although the chemical extraction of biofunctional molecules from different fruits and cereals have been studied extensively, the impact of food processing and digestion on bioactivity has not been studied systematically. The aim of this study was to investigate the biofunctional potential of blackcurrant powder incorporated into wholemeal wheat and barley cookies in after simulated in vitro digestion. The incorporation of blackcurrant significantly (p<0.05) increased the total phenolic content (about 60 %), significantly improved oxygen radical absorbance capacity (about 25 %) of the cookie digesta. Additionally cellular antioxidant and anti-proliferative activity (lowest EC₅₀ value 1.02 mg/mL) on human liver cancer cell model, HepG2 was significantly enhanced. Bioactive metabolites of blackcurrant incorporated cookies were significantly supressed the inflammatory cytokine genes IL-1β (about 3-fold), IL-6 (about 0.5-fold) and NF-kB (about 2-fold) regulation and upregulated satiety gene NUCB-2/Nesfatin-1 (about 5-fold) compare with wholemeal wheat and barley control cookies. The exerted synergistic effects of this study suggest that there may be a new and effective option to prevent and control chronic diseases in human.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
