Preprint Article Version 1 This version is not peer-reviewed

1H-NMR metabolite profiles and discriminate analysis of two different kinds of freshwater fish soups before and after in-vitro gastrointestinal digestion

Version 1 : Received: 4 September 2018 / Approved: 5 September 2018 / Online: 5 September 2018 (13:00:17 CEST)

How to cite: Du, H.; Xiang, Y.; Zhang, G.; Liu, H.; Wang, X.; Xiong, S. 1H-NMR metabolite profiles and discriminate analysis of two different kinds of freshwater fish soups before and after in-vitro gastrointestinal digestion. Preprints 2018, 2018090098 (doi: 10.20944/preprints201809.0098.v1). Du, H.; Xiang, Y.; Zhang, G.; Liu, H.; Wang, X.; Xiong, S. 1H-NMR metabolite profiles and discriminate analysis of two different kinds of freshwater fish soups before and after in-vitro gastrointestinal digestion. Preprints 2018, 2018090098 (doi: 10.20944/preprints201809.0098.v1).

Abstract

Soups show diverse health functions, which should be related with their nutrient profiles. NMR spectroscopy is a robust, sensitive and rapid method to unveil or identify the chemical composition of food or food-derived metabolites. In the current study, 1H-NMR spectroscopy approach was applied to identify the metabolic profiling of two kinds of home-cooked freshwater fish soups (crucian carp and snakehead fish) before and after in vitro gastrointestinal digestion. The nutritional profiles of these two kinds of soups were identified at first. With the help of OPLS-DA method (Orthogonal Partial Least Squares Discriminant Analysis), these two different kinds freshwater fish under different states were completely discriminated. The metabolites changes in digested fish soups could reveal the information of chemical compounds which play important roles in the body. Furthermore, the metabolic patterns of different kinds of fish soups could reflect the various nutrition profiling characteristics for dietary therapy.

Subject Areas

Freshwater fish; Soup; Metabolites; 1H-NMR; In vitro digestion;

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