Preprint Article Version 1 This version is not peer-reviewed

Selective Uropathogenic E. coli Detection using Crossed Surface Relief Gratings

Version 1 : Received: 31 August 2018 / Approved: 1 September 2018 / Online: 1 September 2018 (17:29:04 CEST)

A peer-reviewed article of this Preprint also exists.

Nair, S.; Gomez-Cruz, J.; Manjarrez-Hernandez, Á.; Ascanio, G.; Sabat, R.G.; Escobedo, C. Selective Uropathogenic E. coli Detection Using Crossed Surface-Relief Gratings. Sensors 2018, 18, 3634. Nair, S.; Gomez-Cruz, J.; Manjarrez-Hernandez, Á.; Ascanio, G.; Sabat, R.G.; Escobedo, C. Selective Uropathogenic E. coli Detection Using Crossed Surface-Relief Gratings. Sensors 2018, 18, 3634.

Journal reference: Sensors 2018, 18, 3634
DOI: 10.3390/s18113634

Abstract

Given the rise in the number of cases and their recurrences, Urinary Tract Infections (UTI) are one of the major burdens on public healthcare worldwide. Rapid, inexpensive and selective detection of Uropathogenic E. coli (UPEC), a major contributor to UTIs, is the need of the hour for effective treatment, given the rise of antibiotic-resistant bacteria due to improper diagnosis. Here we present a rapid, real-time, selective and label-free detection of UPEC using an integrated sensing platform based on Crossed Surface Relief Gratings (CSRGs) as nanoplasmonic sensors. Detection is achieved due to the unique Surface Plasmon Resonance (SPR)-based light energy exchange attributed to the CSRGs, allowing real-time sensing in a very narrow bandwidth of the incident light to pass where the SPR energy conversion occurs. The sensing ability of the platform is experimentally demonstrated by the detection of bulk Refractive Index (RI) changes, with a bulk sensitivity of 382.2 nm/RIU and a resolution in the order of 10-6 RIU. We demonstrate selective capture and detection of clinical concentration of UPEC, as opposed to other gram-negative bacteria, in real-time, a first for CSRGs. This work is particularly important for effective treatment of UTIs, allowing point-of-care diagnosis for economically disadvantaged regions around the world.

Subject Areas

surface plasmon resonance; urinary tract infection; surface relief gratings; crossed surface relief gratings; nano-plasmonics; biosensing; uropathogenic E. coli

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