Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Effects of Amelogenin Exon 5 Encoded Peptide from Enamel Matrix Derivative Enhances in Odontoblast like Cells, KN-3 Cells

Hirohito Kato
,
Yoichiro Taguchi
* ,
Kazuya Tominaga
,
Kazutaka Imai
ORCID logo ,
Yaru Ruan
,
Masahiro Noguchi
,
Yu-wei Tsai
,
Yi-Chie Chen
,
Muneyasu Shida
,
Reiko Taguchi
,
Hiroshi Maeda
,
Akio Tanaka
,
Makoto Umeda
Version 1 : Received: 30 August 2018 / Approved: 30 August 2018 / Online: 30 August 2018 (13:01:52 CEST)

A peer-reviewed article of this Preprint also exists.

Kato, H.; Taguchi, Y.; Imai, K.; Ruan, Y.; Tsai, Y.-W.; Chen, Y.-C.; Shida, M.; Taguchi, R.; Tominaga, K.; Umeda, M. The Enhancing Effects of Amelogenin Exon 5-Encoded Peptide from Enamel Matrix Derivative on Odontoblast-Like KN-3 Cells. Appl. Sci. 2018, 8, 1890. Kato, H.; Taguchi, Y.; Imai, K.; Ruan, Y.; Tsai, Y.-W.; Chen, Y.-C.; Shida, M.; Taguchi, R.; Tominaga, K.; Umeda, M. The Enhancing Effects of Amelogenin Exon 5-Encoded Peptide from Enamel Matrix Derivative on Odontoblast-Like KN-3 Cells. Appl. Sci. 2018, 8, 1890.

Abstract

Enamel matrix derivative (EMD) is used for periodontal tissue regeneration therapy. We designed a synthetic amelogenin peptide (SP) derived from EMD, and have previously investigated the biological function of SP. However, it is unknown whether SP affects odontoblastic differentiation. In the present study, we investigated the effects of SP in odontoblast-like cells, KN-3 cells. KN-3 cells were treated with SP (0, 1, 10, 100, or 1000 ng/mL) and then cultured for 3, 8, 24, or 48 hours, in order to determine the effects of SP on cell proliferation and detect its optimum concentration. To investigate the effect of SP on odontogenic differentiation, KN-3 cells were treated with SP in odontogenic differentiation medium cultured for 3 or 7 days. Odontogenic differentiation was performed by measuring alkaline phosphatase (ALP) activity, the mRNA expression of dentin sialophosphoprotein (DSPP), the formation of calcified nodules, and calcium deposition into the extracellular matrix. The addition of SP significantly promoted KN-3 cell proliferation; a concentration of 100 ng/ml generated the greatest change in cell proliferation. SP also showed increased expression of markers of odontogenic differentiation and mineralization. These results suggest that SP, derived from EMD, could be a potential for applicate to the dental pulp capping.

Keywords

emdogain; amelogenin; odontoblast; differentiation; mineralization

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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