Preprint Review Version 1 This version is not peer-reviewed

The Applications of Promoter-Gene Engineered Bio-Sensors

Version 1 : Received: 15 July 2018 / Approved: 18 July 2018 / Online: 18 July 2018 (10:22:39 CEST)

A peer-reviewed article of this Preprint also exists.

Feng, Y.; Xie, Z.; Jiang, X.; Li, Z.; Shen, Y.; Wang, B.; Liu, J. The Applications of Promoter-gene-Engineered Biosensors. Sensors 2018, 18, 2823. Feng, Y.; Xie, Z.; Jiang, X.; Li, Z.; Shen, Y.; Wang, B.; Liu, J. The Applications of Promoter-gene-Engineered Biosensors. Sensors 2018, 18, 2823.

Journal reference: Sensors 2018, 18, 2823
DOI: 10.3390/s18092823

Abstract

Promoter is a small region of DNA sequence in response to various transcription factors, which initiates a particular gene expression. The promoter-engineered bio-sensor can activate or repress gene expression through transcription factor recognizing specific molecules, such as polyamine, sugars, lactams, amino acids, organic acids or redox molecule, however, the reported applications of promoter enhanced bio-sensor are not too much. This review paper highlights the strategies of construction of promoter-gene engineered bio-sensor with human and bacteria’s genetic promoter array for high-throughput screening (HTS) molecular drugs, study of membrane protein’s localization and nucleocytoplasmic shuttling mechanism of regulating factor, enzyme activity, detection of the toxicity of intermediate chemicals, and probing bacteria density to improve value-added product titer. These bio-sensors’s sensitivity and specificity can be further improved by proposed approaches of Mn2+ and Mg2+ added random Error-prone PCR and site-directed mutagenesis which is applied for construction of bacteria’s “mutant library”. It is expected to establish flexible HTS platform (Bio-sensor array) to large-scale screen transcription factor-acting drugs, reducing the toxicity of intermediate compounds, and constructing gene dynamic regulatory system in “push and pull” mode to effectively regulate the valuable medicinal product production. This proposed novel promoter-engineered biosensors aided synthetic genetic circuit construction will maximize the efficiency of bio-synthesis of medicinal compound, which will greatly promote the development of microbial metabolic engineering and biomedical science.

Subject Areas

bio-sensor; promoter; sensitivity; specificity; high-throughput screening (HTS); genetic promoter chip; “push and pull” mode; toxicity

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