Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effects of Boswellia serrata Roxb. and Curcuma longa L. in an In Vitro Intestinal Inflammation Model Using Immune Cells and Caco-2

Paolo Governa
* ORCID logo ,
Maddalena Marchi
,
Veronica Cocetta
,
Bianca De Leo
,
Philippa T. K. Saunders
ORCID logo ,
Daniela Catanzaro
,
Monica Montopoli
,
Marco Biagi
ORCID logo
Version 1 : Received: 17 July 2018 / Approved: 18 July 2018 / Online: 18 July 2018 (07:51:12 CEST)
Version 2 : Received: 10 October 2018 / Approved: 11 October 2018 / Online: 11 October 2018 (05:22:37 CEST)
Version 3 : Received: 7 November 2018 / Approved: 8 November 2018 / Online: 8 November 2018 (04:53:46 CET)

How to cite: Governa, P.; Marchi, M.; Cocetta, V.; De Leo, B.; Saunders, P.T.K.; Catanzaro, D.; Montopoli, M.; Biagi, M. Effects of Boswellia serrata Roxb. and Curcuma longa L. in an In Vitro Intestinal Inflammation Model Using Immune Cells and Caco-2. Preprints 2018, 2018070317. https://doi.org/10.20944/preprints201807.0317.v1. Governa, P.; Marchi, M.; Cocetta, V.; De Leo, B.; Saunders, P.T.K.; Catanzaro, D.; Montopoli, M.; Biagi, M. Effects of Boswellia serrata Roxb. and Curcuma longa L. in an In Vitro Intestinal Inflammation Model Using Immune Cells and Caco-2. Preprints 2018, 2018070317. https://doi.org/10.20944/preprints201807.0317.v1.

Abstract

Inflammatory bowel diseases, which consist of chronic inflammatory conditions of the colon and the small intestine, are considered a global disease of our modern society. Recently, the use of herbal therapies has increased in patients with inflammatory bowel diseases because of their effectiveness and better safety profile, compared to conventional drugs. Boswellia serrata Roxb. and Curcuma longa L. are amongst the most promising herbal drugs, however, their clinical use in inflammatory bowel diseases is limited and little is known on their mechanism of action. The aim of this work was to investigate the effects of two standardized extract of B. serrata and C. longa in an in vitro model of intestinal inflammation. Their impact on cytokine release and reactive oxygen species production, as well as the maintenance of the intestinal barrier function and on intestinal mucosa immune cells infiltration, has been evaluated. The extracts showed a good protective effect on the intestinal epithelium at 1 µg/ml, with C. longa having an anti-inflammatory mechanism of action and B. serrata acting as an antioxidant. In summary, these herbal products were demonstrated to be promising agents for the management of inflammatory bowel diseases by modulating in vitro parameters which have been identified in the clinical conditions.

Keywords

Boswellia serrata Roxb.; Curcuma longa L.; intestinal bowel diseases (IBD); Caco-2; PBMC; HMC-1.1; mast cells; cytokines; trans epithelial electrical resistance (TEER); reactive oxygen species (ROS)

Subject

LIFE SCIENCES, Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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