Preserved in Portico This version is not peer-reviewed
Engineering a Minimal Cloning Vector from a pUC18 Plasmid Backbone with an Extended Multiple Cloning Site
: Received: 16 July 2018 / Approved: 16 July 2018 / Online: 16 July 2018 (12:52:38 CEST)
: Received: 12 October 2018 / Approved: 15 October 2018 / Online: 15 October 2018 (09:22:22 CEST)
A peer-reviewed article of this Preprint also exists.
Journal reference: Biotechniques 2019
Minimal plasmids play an essential role in many intermediate steps in molecular biology. They can for example be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis methods. A small backbone also opens up for additional unique restriction enzyme cloning sites. Here we describe the generation of pICOz, a 1185 bp fully functional high-copy cloning plasmid with an extended multiple cloning site (MCS). To our knowledge, this is the smallest high-copy cloning vector ever described.
PCR; recombination; cloning; engineering; biotechnology; synthetic biology; synthetic nucleotide; plasmids; repository; minimalism; Escherichia coli; mutagenesis
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