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Effects of Rhizome Extract of Dioscorea Batatas and Its Active Compound, Allantoin, on the Regulation of Myoblast Differentiation and Mitochondrial Biogenesis in C2C12 Myotubes
Version 1
: Received: 25 June 2018 / Approved: 25 June 2018 / Online: 25 June 2018 (16:32:43 CEST)
How to cite:
Ma, J.N.; Kang, S.Y.; Park, J.H.; Park, Y.; Jung, H.W. Effects of Rhizome Extract of Dioscorea Batatas and Its Active Compound, Allantoin, on the Regulation of Myoblast Differentiation and Mitochondrial Biogenesis in C2C12 Myotubes. Preprints2018, 2018060398. https://doi.org/10.20944/preprints201806.0398.v1
Ma, J.N.; Kang, S.Y.; Park, J.H.; Park, Y.; Jung, H.W. Effects of Rhizome Extract of Dioscorea Batatas and Its Active Compound, Allantoin, on the Regulation of Myoblast Differentiation and Mitochondrial Biogenesis in C2C12 Myotubes. Preprints 2018, 2018060398. https://doi.org/10.20944/preprints201806.0398.v1
Ma, J.N.; Kang, S.Y.; Park, J.H.; Park, Y.; Jung, H.W. Effects of Rhizome Extract of Dioscorea Batatas and Its Active Compound, Allantoin, on the Regulation of Myoblast Differentiation and Mitochondrial Biogenesis in C2C12 Myotubes. Preprints2018, 2018060398. https://doi.org/10.20944/preprints201806.0398.v1
APA Style
Ma, J.N., Kang, S.Y., Park, J.H., Park, Y., & Jung, H.W. (2018). Effects of Rhizome Extract of <em>Dioscorea Batatas </em>and Its Active Compound, Allantoin, on the Regulation of Myoblast Differentiation and Mitochondrial Biogenesis in C2C12 Myotubes. Preprints. https://doi.org/10.20944/preprints201806.0398.v1
Chicago/Turabian Style
Ma, J.N., Yong-Ki Park and Hyo Won Jung. 2018 "Effects of Rhizome Extract of <em>Dioscorea Batatas </em>and Its Active Compound, Allantoin, on the Regulation of Myoblast Differentiation and Mitochondrial Biogenesis in C2C12 Myotubes" Preprints. https://doi.org/10.20944/preprints201806.0398.v1
Abstract
The present study was conducted to investigate the effects of rhizome extract of Dioscorea batatas (Dioscoreae Rhizoma, Chinese Yam) and its bioactive compound, allantoin, on myoblast differentiation and mitochondrial biogenesis in skeletal muscle cells. Yams were extracted in water and the extract was analyzed by HPLC. The expression of C2C12 myotubes differentiation and mitochondrial biogenesis regulators were determined by reverse transcriptase (RT)-PCR or Western blot. The glucose levels and total ATP contents were determined by glucose consumption, glucose uptake and ATP assays, respectively. Treatment with yam extract (1 mg/mL) and allantoin (0.2 and 0.5 mM) significantly increased of MyHC expression compared with non-treated myotubes. Yam extract and allantoin significantly increased the expression of mitochondrial biogenesis regulating proteins, PGC1?, Sirt-1, NRF-1, and TFAM, as well as the phosphorylation of AMPK and ACC in C2C12 myotubes. Furthermore, yam extract and allantoin significantly increased the glucose uptake levels and the ATP contents. Finally, HPLC analysis revealed that the yam extract contained 1.53% of allantoin. Yam extract and allantoin, stimulated myoblast differentiation into myotubes and increased energy production through upregulation of mitochondrial biogenesis regulators. These findings indicate that yam extract and allantoin can help to prevent the skeletal muscle dysfunction through stimulation of energy metabolism.
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
