Preprint Article Version 1 This version is not peer-reviewed

Tracking Healing Process of Experimental Liver Injuries Treated with Different Sealants and Adhesives Biomaterials. Matrix Metalloproteinase Evaluation

Version 1 : Received: 20 June 2018 / Approved: 21 June 2018 / Online: 21 June 2018 (06:15:23 CEST)
Version 2 : Received: 26 June 2018 / Approved: 27 June 2018 / Online: 27 June 2018 (10:43:43 CEST)

How to cite: Lloris Carsí, J.M.; Barrios-Pitarque, C.; Lloris-Cejalvo, J.M.; Gil-Santos, L.; Cejalvo-Lapeña, D. Tracking Healing Process of Experimental Liver Injuries Treated with Different Sealants and Adhesives Biomaterials. Matrix Metalloproteinase Evaluation. Preprints 2018, 2018060334 (doi: 10.20944/preprints201806.0334.v1). Lloris Carsí, J.M.; Barrios-Pitarque, C.; Lloris-Cejalvo, J.M.; Gil-Santos, L.; Cejalvo-Lapeña, D. Tracking Healing Process of Experimental Liver Injuries Treated with Different Sealants and Adhesives Biomaterials. Matrix Metalloproteinase Evaluation. Preprints 2018, 2018060334 (doi: 10.20944/preprints201806.0334.v1).

Abstract

Sealants and adhesives are used in the repair and preservation of damaged solid organs. This study examines the matrix metalloproteinases (MMP) activity in the healing of liver injuries treated with two biological adhesives (Tachosil® and GelitaSpon®) as well as that of a new elastic cyanoacrylate (Adhflex®). Methods. We induced in 90 male rats hepatic lesions using a Stiefel biopsy punch in the liver. Wound healing was assessed 2, 6, and 18 days after injury by quantifying MMP1, 2, 8, 9, and 13 tissue levels. The histopathological repair was evaluated by hematoxylin-eosin, Masson’s trichrome, and Periodic Acid Schiff (PAS) staining and CD31, CD68 immunohistochemical marker. The three sealants used supported complete healing of liver lesions. Both histopathology and MMP findings indicate that the degradation process of Adhflex® is slower and produces a strong inflammatory reaction at the beginning of healing. Results. All the MMPs measured disclosed higher values at an early stage of the healing process in animals treated with Adhflex® and Tachosil, the expression of for MMP2 and MMP9 being significantly higher in the Adhflex-treated group. Animals treated with Tachosil had significant higher values of MMP8 and MPP13 than the Adhflex-treated group. Animals treated with Adhflex® showed a maintained overexpression in all the MMPs tested even at the latest wound healing stages. Conclusion. Notably, this MMPs overexpression did not negatively influence the histological healing process of hepatic injuries. Given that all hepatic trauma injuries should be considered emergencies, any easy-to-use and rapid sealant, such as Adhflex®, could be considered a suitable treatment option.

Subject Areas

hepatic injury; sealants; metalloproteinases; inflammatory response; wound healing

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