Preprint Article Version 1 This version is not peer-reviewed

The Endochitinase of Clonostachys Rosea Enhances the Biocontrol Efficiency of Bacillus Amyloliquefaciens by Increasing Its Activities of Defense Enzymes

Version 1 : Received: 16 June 2018 / Approved: 19 June 2018 / Online: 19 June 2018 (15:56:47 CEST)

A peer-reviewed article of this Preprint also exists.

Zheng, Y.; Wang, X.; Liu, S.; Zhang, K.; Cai, Z.; Chen, X.; Zhang, Y.; Liu, J.; Wang, A. The Endochitinase of Clonostachysrosea Expression in Bacillus amyloliquefaciens Enhances the Botrytis cinerea Resistance of Tomato. Int. J. Mol. Sci. 2018, 19, 2221. Zheng, Y.; Wang, X.; Liu, S.; Zhang, K.; Cai, Z.; Chen, X.; Zhang, Y.; Liu, J.; Wang, A. The Endochitinase of Clonostachysrosea Expression in Bacillus amyloliquefaciens Enhances the Botrytis cinerea Resistance of Tomato. Int. J. Mol. Sci. 2018, 19, 2221.

Journal reference: Int. J. Mol. Sci. 2018, 19, 2221
DOI: 10.3390/ijms19082221

Abstract

To investigate whether the ech42 gene in Clonostachys rosea can improve the biocontrol efficacy of Bacillus amyloliquefaciens and its molecular mechanism.Compared to the wild type, the B. amyloliquefaciens transformed with the ech42 gene exhibited a higher chitinase activity. The B. amyloliquefaciens-ech42 also showed a significantly higher biocontrol efficiency against B. cinerea when tomato plants were pre-treated with amyloliquefaciens-ech42. No significant difference of control efficiency was observed between the wild type and amyloliquefaciens-ech42 when tomato plants were first infected by B. cinerea. In addition, the activity of the defense-related enzyme polyphenol oxidase, but not superoxide dismutase was significantly higher in amyloliquefaciens-ech42 than in the wild type.The ech42 enhances the Bacillus amyloliquefaciens biocontrol efficiency by increasing the capacity of protection/prevention to plants, rather treating/killing the pathogens.

Subject Areas

Keywords: Bacillus amyloliquefaciens, Chitinase, Defense enzymes; Biological control

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