Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Endochitinase of Clonostachys Rosea Enhances the Biocontrol Efficiency of Bacillus Amyloliquefaciens by Increasing Its Activities of Defense Enzymes

Yangyang Zheng
ORCID logo ,
Xudong Wang
,
Siyuan Liu
,
Kewei Zhang
,
Zhibo Cai
,
Xiuling Chen
,
Yao Zhang
,
Jiayin Liu
,
Aoxue Wang
*
Version 1 : Received: 16 June 2018 / Approved: 19 June 2018 / Online: 19 June 2018 (15:56:47 CEST)

A peer-reviewed article of this Preprint also exists.

Zheng, Y.; Wang, X.; Liu, S.; Zhang, K.; Cai, Z.; Chen, X.; Zhang, Y.; Liu, J.; Wang, A. The Endochitinase of Clonostachysrosea Expression in Bacillus amyloliquefaciens Enhances the Botrytis cinerea Resistance of Tomato. Int. J. Mol. Sci. 2018, 19, 2221. Zheng, Y.; Wang, X.; Liu, S.; Zhang, K.; Cai, Z.; Chen, X.; Zhang, Y.; Liu, J.; Wang, A. The Endochitinase of Clonostachysrosea Expression in Bacillus amyloliquefaciens Enhances the Botrytis cinerea Resistance of Tomato. Int. J. Mol. Sci. 2018, 19, 2221.

Abstract

To investigate whether the ech42 gene in Clonostachys rosea can improve the biocontrol efficacy of Bacillus amyloliquefaciens and its molecular mechanism.Compared to the wild type, the B. amyloliquefaciens transformed with the ech42 gene exhibited a higher chitinase activity. The B. amyloliquefaciens-ech42 also showed a significantly higher biocontrol efficiency against B. cinerea when tomato plants were pre-treated with amyloliquefaciens-ech42. No significant difference of control efficiency was observed between the wild type and amyloliquefaciens-ech42 when tomato plants were first infected by B. cinerea. In addition, the activity of the defense-related enzyme polyphenol oxidase, but not superoxide dismutase was significantly higher in amyloliquefaciens-ech42 than in the wild type.The ech42 enhances the Bacillus amyloliquefaciens biocontrol efficiency by increasing the capacity of protection/prevention to plants, rather treating/killing the pathogens.

Keywords

Keywords: Bacillus amyloliquefaciens, Chitinase, Defense enzymes; Biological control

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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