Preprint Article Version 1 This version is not peer-reviewed

The Use of Colors as an Alternative to Size in Mold Growth Studies

Version 1 : Received: 28 May 2018 / Approved: 28 May 2018 / Online: 28 May 2018 (16:31:22 CEST)

How to cite: Cambaza, E.; Koseki, S.; Kawamura, S. The Use of Colors as an Alternative to Size in Mold Growth Studies. Preprints 2018, 2018050415 (doi: 10.20944/preprints201805.0415.v1). Cambaza, E.; Koseki, S.; Kawamura, S. The Use of Colors as an Alternative to Size in Mold Growth Studies. Preprints 2018, 2018050415 (doi: 10.20944/preprints201805.0415.v1).

Abstract

Size-based fungal growth studies have limitations. For example, the growth in size stops in closed systems once it reaches the borders and poorly describes the metabolic status, especially in the stationary phase. This might lead mycotoxin studies to unrealistic results. Color change could be a viable alternative as pigments are results of the mold’s metabolic activity. This study aimed to verify the possibility of using gray values and the RGB system to analyze the growth of Fusarium graminearum. It consisted color and area measurement using the ImageJ software for specimens grown in yeast extract agar (YEA). The results suggest the usability of color and gray values as reliable tools to analyze the growth of F. graminearum.

Subject Areas

Fusarium graminearum, mycelial growth, RGB, gray scale.

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