PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
New Methodology for Rapid Formation and Prevention of Advanced Glycation end Products (AGEs) In Vitro Coupled with the Hypoxanthine/Xanthine Oxidase Assay System
Version 1
: Received: 27 May 2018 / Approved: 28 May 2018 / Online: 28 May 2018 (13:28:19 CEST)
How to cite:
Marques, S.; Trevisan, T.; Maia, C.; Breuer, A.; Owen, R. New Methodology for Rapid Formation and Prevention of Advanced Glycation end Products (AGEs) In Vitro Coupled with the Hypoxanthine/Xanthine Oxidase Assay System. Preprints2018, 2018050409. https://doi.org/10.20944/preprints201805.0409.v1
Marques, S.; Trevisan, T.; Maia, C.; Breuer, A.; Owen, R. New Methodology for Rapid Formation and Prevention of Advanced Glycation end Products (AGEs) In Vitro Coupled with the Hypoxanthine/Xanthine Oxidase Assay System. Preprints 2018, 2018050409. https://doi.org/10.20944/preprints201805.0409.v1
Marques, S.; Trevisan, T.; Maia, C.; Breuer, A.; Owen, R. New Methodology for Rapid Formation and Prevention of Advanced Glycation end Products (AGEs) In Vitro Coupled with the Hypoxanthine/Xanthine Oxidase Assay System. Preprints2018, 2018050409. https://doi.org/10.20944/preprints201805.0409.v1
APA Style
Marques, S., Trevisan, T., Maia, C., Breuer, A., & Owen, R. (2018). New Methodology for Rapid Formation and Prevention of Advanced Glycation end Products (AGEs) In Vitro Coupled with the Hypoxanthine/Xanthine Oxidase Assay System. Preprints. https://doi.org/10.20944/preprints201805.0409.v1
Chicago/Turabian Style
Marques, S., Andrea Breuer and Robert Owen. 2018 "New Methodology for Rapid Formation and Prevention of Advanced Glycation end Products (AGEs) In Vitro Coupled with the Hypoxanthine/Xanthine Oxidase Assay System" Preprints. https://doi.org/10.20944/preprints201805.0409.v1
Abstract
Advanced Glycation End Products (AGEs) represent a set of substances that contribute directly to the triggering and/or aggravation of pathologies associated with ageing. AGEs are produced by the reaction between reducing sugars (or α-dicarbonyl compounds) proteins and amino acid residues. Current methodologies require an incubation period of 1-3 weeks to generate AGEs. In this study the reaction time for the formation of AGEs (48 and 3 hours) is significantly reduced by coupling and adapting procedures already existing in the literature to the free radical generation system called the hypoxanthine/xanthine oxidase assay. The capacity of different classes and chemical compounds (aminoguanidine, chlorogenic acid, rutin, extracts of Hancornia speciosa Gomes) were evaluated to inhibit the protein glycation process, acting as capturing agents of α-dicarbonyl species. Aminoguanidine, rutin and the leaf extracts of Hancornia speciosa Gomes show a high capacity to act as α-dicarbonyl compound scavengers (RCS-trapping) and resulting in the inhibition of AGEs formation.
Keywords
advanced glycation end products; glyoxal; hypoxanthine/xanthine oxidase; methyl glyoxal; RCS-trapping
Subject
Medicine and Pharmacology, Pathology and Pathobiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
