Preprint Article Version 1 This version is not peer-reviewed

Evolutionary History of the GH3 Family Based on 48 Sequenced Plants and Identification of Jasmonic Acid-Relate GH3 Genes in Solanum tuberosum

Version 1 : Received: 16 May 2018 / Approved: 17 May 2018 / Online: 17 May 2018 (12:01:00 CEST)

How to cite: Zhang, C.; Zhang, L.; Wang, D.; Ma, H.; Liu, B.; Shi, Z.; Ma, X.; Chen, Y.; Chen, Q. Evolutionary History of the GH3 Family Based on 48 Sequenced Plants and Identification of Jasmonic Acid-Relate GH3 Genes in Solanum tuberosum. Preprints 2018, 2018050244 (doi: 10.20944/preprints201805.0244.v1). Zhang, C.; Zhang, L.; Wang, D.; Ma, H.; Liu, B.; Shi, Z.; Ma, X.; Chen, Y.; Chen, Q. Evolutionary History of the GH3 Family Based on 48 Sequenced Plants and Identification of Jasmonic Acid-Relate GH3 Genes in Solanum tuberosum. Preprints 2018, 2018050244 (doi: 10.20944/preprints201805.0244.v1).

Abstract

Glycoside Hydrolase 3 (GH3) is a phytohormone-responsive family of genes that has been found in many plant species. It is implicated in the biological activity of indolacetic (IAA) and jasmonic acids (JA), and also affects plant growth and developmental processes and some stresses. In this study, GH3 genes were identified in 48 plants, which belong to algae, moss, fern, gymnosperm and angiosperm. No GH3 representative gene has been found in algae, and our research identified 4 genes in mosses, 19 in ferns, 7 in gymnosperms, and numerous in Angiosperms. The results showed that GH3 genes mainly occur in seed plants. Phylogenetic analysis of all GH3 genes showed three separate clades. Group I was related to JA adenylation, group II was related to IAA adenylation, and group III was separated from group II but the function was not clear. The structure of GH3 protein indicated highly conserved sequence in the plant kingdom. The analysis of JA-adenylation related to gene expression of GH3 in potato (Solanum tuberosum) showed that StGH3.12 highly responded to Methyl Jasmonate (MeJA) treatment. Expression levels of StGH3.1, StGH3.11, and StGH3.12 were high in flower and StGH3.11 expression was also high in stolon. Our research revealed the evolution of the GH3 family, which is useful for studying the precise function about JA-adenylation GH3 genes in S. tuberosum under development and biotic stresses.

Subject Areas

GH3 family; sequencing plants; potato; jasmonic acids; tissues; biotic

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