Preprint Article Version 1 This version is not peer-reviewed

Matrix Metalloproteinase Expression during the Healing Process of Liver Injuries Treated with Different Biological Sealants and Adhesives

Version 1 : Received: 19 April 2018 / Approved: 20 April 2018 / Online: 20 April 2018 (11:14:49 CEST)

How to cite: Lloris-Carsí, J.M.; Barrios, C.; Lloris-Cejalvo, J.M.; Lloris-Salvi, M.; Cejalvo-Lapeña, D. Matrix Metalloproteinase Expression during the Healing Process of Liver Injuries Treated with Different Biological Sealants and Adhesives. Preprints 2018, 2018040262 (doi: 10.20944/preprints201804.0262.v1). Lloris-Carsí, J.M.; Barrios, C.; Lloris-Cejalvo, J.M.; Lloris-Salvi, M.; Cejalvo-Lapeña, D. Matrix Metalloproteinase Expression during the Healing Process of Liver Injuries Treated with Different Biological Sealants and Adhesives. Preprints 2018, 2018040262 (doi: 10.20944/preprints201804.0262.v1).

Abstract

Background. Adhesives and sealants can be used to repair and preserve solid damaged organs. This study explores the activity of different matrix metalloproteinases (MMP) during the healing of liver injuries treated with two biological adhesives (Tachosil and GelitaSpon) and a new synthetic elastic cyanoacrylate (Adhflex®). Methods. Liver traumatic injuries were experimentally induced in 90 male Wistar rats using a Stiefel biopsy punch in the liver. Wound healing was evaluated 2, 6, and 18 days after injury by determining MMP1, 2, 8, 9, and 13 expression. The histopathological repair was assessed by hematoxylin-eosin, Masson’s trichrome, and Periodic Acid Schiff (PAS) staining. The three sealants used supported complete healing of the liver lesions. Both histopathology and MMP findings indicate that the degradation process of Adhflex® is slower and produces a strong initial inflammatory reaction. Results. All the MMPs measured disclosed higher values at early stage of the healing process in animals treated with Adhflex® and Tachosil, being the expression of for MMP2 and MMP9 significantly higher in the Adhflex-treated group. Animals treated with Tachosil had significant higher values of MMP8 and MPP13 than the Adhflex-treated group. Animals treated with Adhflex® showed a maintained overexpression in all the MMPs tested even at the latest wound healing stages. Conclusion. Notably, this MMPs overexpression did not influence negatively the histological healing process of the hepatic injuries. Given that all hepatic trauma injuries should be considered emergencies, any easy-to-use and rapid sealant, such as Adhflex®, could be considered as a suitable treatment option.

Subject Areas

hepatic injury; sealants; metalloproteinases; inflammatory response; wound healing

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