Preprint Article Version 1 This version is not peer-reviewed

Enzymatic Bioconversion of Cholesterol Using Glucosyltransferase and Methyltransferase Isolated from Streptomyces

Version 1 : Received: 18 April 2018 / Approved: 19 April 2018 / Online: 19 April 2018 (09:31:06 CEST)

How to cite: Koirala, N. Enzymatic Bioconversion of Cholesterol Using Glucosyltransferase and Methyltransferase Isolated from Streptomyces. Preprints 2018, 2018040247 (doi: 10.20944/preprints201804.0247.v1). Koirala, N. Enzymatic Bioconversion of Cholesterol Using Glucosyltransferase and Methyltransferase Isolated from Streptomyces. Preprints 2018, 2018040247 (doi: 10.20944/preprints201804.0247.v1).

Abstract

A sterol glycosyltransferases (SGT) gene (sav7185; accession number NP_828361) was isolated from Streptomyces avermitilis MA-4680. The full-length gene consists of 1284 nucleotides and encodes 427 amino acids with a calculated mass of 46.05 kDa. The gene was then cloned in pET28a vector and heterologously expressed in Escherichia coli BL21 (DE3) and was used for the biotransformation of cholesterol. This SGT protein showed favorable activity towards cholesterol tested in this study. Further, we tested the conversion of cholesterol to its methoxide using another Streptomyces O-methyltransferase (accession number KF420279). This O-methyltransferase (OMT) SpOMT2884, originating from Streptomyces peucetius ATCC 27952, was cloned, expressed, and applied for the production of methylated derivative. The GC-MS analysis confirmed the conversion of cholesterol into cholesterol-3-O-β-D-glucoside and a novel cholesterol-3-O-methoxide. Hence, these Streptomyces SGT and OMT could find applications for the derivatization of pharmaceutically significant sterols.

Subject Areas

sterol glycosyltransferase; O-methyltransferase; Streptomyces avermitilis MA-4680; biotransformation; Cholesterol-3-O-β-D-glucoside; GC-MS

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