Preprint Article Version 1 This version is not peer-reviewed

Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers

Version 1 : Received: 7 April 2018 / Approved: 9 April 2018 / Online: 9 April 2018 (10:48:47 CEST)

A peer-reviewed article of this Preprint also exists.

Zhou, W.; Wang, Y.; Zhang, G.; Luan, G.; Chen, S.; Meng, J.; Wang, H.; Hu, N.; Suo, Y. Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers. Molecules 2018, 23, 1048. Zhou, W.; Wang, Y.; Zhang, G.; Luan, G.; Chen, S.; Meng, J.; Wang, H.; Hu, N.; Suo, Y. Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers. Molecules 2018, 23, 1048.

Journal reference: Molecules 2018, 23, 1048
DOI: 10.3390/molecules23051048

Abstract

The dioecious property of sea buckthorn (Hippophae rhamnoides L.) prevents sex recognition via traditional observation at the juvenile stage, thus impeding breeding and economic cropping; RAPD and SCAR markers were used to identify the sexes. A total of 45 random decamer primers were used to screen genomic DNA pools of staminate and pistillate genotypes for genetic polymorphisms. One female sex-linked marker was identified. D15 (5′-CATCCGTGCT-3′) amplified a particular band of 885 bp, which showed polymorphism among staminate and pistillate genotype plants. The SCAR marker Hrcx-15 was obtained by sequencing the fragment. The alleles of 140 pistillate genotypes were examined but not of the 140 staminate genotypes discerned via taxonomy. Staminate and pistillate genotypes of sea buckthorn plants can be distinguished, using Hrcx-15 as a genetic marker for sex identification and for expediting cultivation for commercial applications.

Subject Areas

Hippophae rhamnoides L.; dioecious; sex determination; RAPD; SCAR

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