Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers

Wu Zhou; Yuwei Wang; Gong Zhang; Guangxiang Luan; Shasha Chen; Jing Meng; Honglun Wang; Na Hu; Yourui Suo

doi:10.20944/preprints201804.0107.v1

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preprints.org > biology > plant sciences > doi: 10.20944/preprints201804.0107.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers

^* ,

Version 1 : Received: 7 April 2018 / Approved: 9 April 2018 / Online: 9 April 2018 (10:48:47 CEST)

A peer-reviewed article of this Preprint also exists.

Zhou, W.; Wang, Y.; Zhang, G.; Luan, G.; Chen, S.; Meng, J.; Wang, H.; Hu, N.; Suo, Y. Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers. Molecules 2018, 23, 1048. Zhou, W.; Wang, Y.; Zhang, G.; Luan, G.; Chen, S.; Meng, J.; Wang, H.; Hu, N.; Suo, Y. Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers. Molecules 2018, 23, 1048.

DOI: 10.3390/molecules23051048

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Abstract

The dioecious property of sea buckthorn (Hippophae rhamnoides L.) prevents sex recognition via traditional observation at the juvenile stage, thus impeding breeding and economic cropping; RAPD and SCAR markers were used to identify the sexes. A total of 45 random decamer primers were used to screen genomic DNA pools of staminate and pistillate genotypes for genetic polymorphisms. One female sex-linked marker was identified. D15 (5′-CATCCGTGCT-3′) amplified a particular band of 885 bp, which showed polymorphism among staminate and pistillate genotype plants. The SCAR marker Hrcx-15 was obtained by sequencing the fragment. The alleles of 140 pistillate genotypes were examined but not of the 140 staminate genotypes discerned via taxonomy. Staminate and pistillate genotypes of sea buckthorn plants can be distinguished, using Hrcx-15 as a genetic marker for sex identification and for expediting cultivation for commercial applications.

Keywords

Hippophae rhamnoides L.; dioecious; sex determination; RAPD; SCAR

Subject

BIOLOGY, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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