Preprint Review Version 1 This version is not peer-reviewed

Formation of Functional Nanobiocatalysts with a Novel and Encouraging Immobilization Approach and Their Versatile Bioanalytical Applications

Version 1 : Received: 31 March 2018 / Approved: 2 April 2018 / Online: 2 April 2018 (08:15:44 CEST)

How to cite: Tasdemir, D.; Celik, C.; Demirbas, A.; Katı, A.; Gul, O.T.; Çimen, B.; Ocsoy, I. Formation of Functional Nanobiocatalysts with a Novel and Encouraging Immobilization Approach and Their Versatile Bioanalytical Applications. Preprints 2018, 2018040011 (doi: 10.20944/preprints201804.0011.v1). Tasdemir, D.; Celik, C.; Demirbas, A.; Katı, A.; Gul, O.T.; Çimen, B.; Ocsoy, I. Formation of Functional Nanobiocatalysts with a Novel and Encouraging Immobilization Approach and Their Versatile Bioanalytical Applications. Preprints 2018, 2018040011 (doi: 10.20944/preprints201804.0011.v1).

Abstract

A recent breakthrough in preparation of immobilized enzyme based biocatalysts achieving highly enhanced enzymatic activities and stabilities has become a great alternative to conventional immobilization techniques. The functional hybrid nanobiocatalysts (FHNs) fabricated in this immobilization composed of organic components (amino acid, peptide, protein, enzyme and plant extract) and inorganic components (various metal ions) give flower-like morphology with narrow size distribution and porous structure. The enzyme incorporated FHNs exhibite greatly enhanced catalytic activities and stabilities compared to free and conventionally immobilized enzymes under various experimental conditions. In addition to that, the FHNs consisting of other organic components act as Fenton-like reagents and show peroxidase-like activity owing to presence of metal ions and porous structure in the FHNs. This report basically focuses on preparation, characterization, and bioanalytical applications of the FHNs and explain mechanism of the FHNs formation and thier enhanced activities and stabilities.

Subject Areas

enzyme; immobilization; organic-inorganic nanoflower; nanobiocatalysts

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