PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Investigation of invitro Antioxidant and invivo Protective Effects of Hypericum triquetrifolium Seed Methanol Extracts against Cyclophosphamide-Induced Acute Myelotoxicity, Hemotoxicity and Hepatotoxicity in Rats
Version 1
: Received: 17 March 2018 / Approved: 19 March 2018 / Online: 19 March 2018 (08:53:25 CET)
How to cite:
Cetik Yildiz, S.; Keskin, C.; Ayhanci, A. Investigation of invitro Antioxidant and invivo Protective Effects of Hypericum triquetrifolium Seed Methanol Extracts against Cyclophosphamide-Induced Acute Myelotoxicity, Hemotoxicity and Hepatotoxicity in Rats. Preprints2018, 2018030148. https://doi.org/10.20944/preprints201803.0148.v1
Cetik Yildiz, S.; Keskin, C.; Ayhanci, A. Investigation of invitro Antioxidant and invivo Protective Effects of Hypericum triquetrifolium Seed Methanol Extracts against Cyclophosphamide-Induced Acute Myelotoxicity, Hemotoxicity and Hepatotoxicity in Rats. Preprints 2018, 2018030148. https://doi.org/10.20944/preprints201803.0148.v1
Cetik Yildiz, S.; Keskin, C.; Ayhanci, A. Investigation of invitro Antioxidant and invivo Protective Effects of Hypericum triquetrifolium Seed Methanol Extracts against Cyclophosphamide-Induced Acute Myelotoxicity, Hemotoxicity and Hepatotoxicity in Rats. Preprints2018, 2018030148. https://doi.org/10.20944/preprints201803.0148.v1
APA Style
Cetik Yildiz, S., Keskin, C., & Ayhanci, A. (2018). Investigation of invitro Antioxidant and invivo Protective Effects of Hypericum triquetrifolium Seed Methanol Extracts against Cyclophosphamide-Induced Acute Myelotoxicity, Hemotoxicity and Hepatotoxicity in Rats. Preprints. https://doi.org/10.20944/preprints201803.0148.v1
Chicago/Turabian Style
Cetik Yildiz, S., Cumali Keskin and Adnan Ayhanci. 2018 "Investigation of invitro Antioxidant and invivo Protective Effects of Hypericum triquetrifolium Seed Methanol Extracts against Cyclophosphamide-Induced Acute Myelotoxicity, Hemotoxicity and Hepatotoxicity in Rats" Preprints. https://doi.org/10.20944/preprints201803.0148.v1
Abstract
The aim of this study was to investigate in-vitro antioxidant properties and in-vivo protective effects of different concentrations of Hypericum triquetrifolium Turra. (HT) seed methanol extracts against acute hepatotoxicity, myelotoxicity and hematotoxicity in rats exposed to overdose of cyclophosphamide (CP). HT seed methanol extracts were tested in view of its in-vitro antioxidant activities as total phenolic contents and DPPH free radical-scavenging activity. To investigate in-vivo protective effects of HT seed methanol extracts on rat tissues; tested animals were divided into nine groups. Three groups only were treated with HT extracts (25, 50 and 100 mg/kg HT) for 6 days. Three groups were pre-treated with the extract of HT (25, 50 and 100 mg/kg HT) for 6 days and on the last day they were injected with single dose of CP (150-mg/kg body weight). Two groups were used as control groups and one group was only treated with CP (150 mg/kg) on the 6th day. The toxic effects of CP and protective effects of HT extracts on the nucleated cells which were produced by bone marrow and serum alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), oxidative stress index (OSI) levels were investigated biochemically. Additionally, liver tissue samples were examined histopathologically. Our results show that HT seed methanol extract has high total phenolic content and antioxidant activity. Over dose CP administration caused hepatotoxicity, myelotoxicity and hematotoxicity on rat. Whereas, 25, 50 and 100 mg/kg HT plus CP administered groups showed significant protective effects on nucleated cells. And 25, 50, 100 mg/kg HT plus CP treated groups showed an important decrease on serum ALT, ALP, LDH and OSI levels when compared with CP treated group. Our results showed that the administration of different HT doses with high doses of CP significantly reduced hepatotoxicity, myelotoxicity and hematoxicity on rats.
Keywords
cyclophosphamide; cytoprotective; hepatotoxicity; hematotoxicity; Hypericum triquetrifolium turra.; myelotoxicity; rat
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
