Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Molecular Diversity of The Tidal Swamp Rice (Oryza sativa L.) Germplasm of The South Kalimantan, Indonesia

Version 1 : Received: 15 March 2018 / Approved: 15 March 2018 / Online: 15 March 2018 (06:50:32 CET)

How to cite: Mursyidin, D.H.; Purnomo, P.; Sumardi, I.; Daryono, B.S. Molecular Diversity of The Tidal Swamp Rice (Oryza sativa L.) Germplasm of The South Kalimantan, Indonesia. Preprints 2018, 2018030120. https://doi.org/10.20944/preprints201803.0120.v1 Mursyidin, D.H.; Purnomo, P.; Sumardi, I.; Daryono, B.S. Molecular Diversity of The Tidal Swamp Rice (Oryza sativa L.) Germplasm of The South Kalimantan, Indonesia. Preprints 2018, 2018030120. https://doi.org/10.20944/preprints201803.0120.v1

Abstract

Tidal swamp rice has long been cultivated by the local people of the South Kalimantan, Indonesia. This germplasm possess some important traits for adapted to a wide range of abiotic and biotic stresses. In this study, a total of sixteen cultivars of this germplasm, consisting of fifteen of the South Kalimantan Province and one of Sumatera Island, Indonesia (an outgroup) were analyzed, phylogenetically based on the chloroplast trnL-F and nuclear intergenic spacer region (IGS). The results showed that this germplasm has a relatively more extraordinary genetic diversity than other local rice germplasm. In a nucleotide level, this germplasm showed a genetic diversity of 0.61 for nuclear IGS and 0.58 for trnL-F. The phylogenetic reconstruction also exhibited that this germplasm has the unique illustration of phylogenetic trees, particularly for the combined sequence datasets. Thus, the results of our study would provide useful information for further understanding of evolutionary relationships of this germplasm and facilitate the efficient utilization of valuable genes for genetic improvement, particularly in the tidal swamp areas.

Keywords

Oryza sativa; genetic diversity; tidal swamp; breeding and conservation programs

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

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