Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

S-layer Protein-based Biosensors

Version 1 : Received: 9 March 2018 / Approved: 9 March 2018 / Online: 9 March 2018 (12:10:28 CET)

How to cite: Schuster, B. S-layer Protein-based Biosensors. Preprints 2018, 2018030071. Schuster, B. S-layer Protein-based Biosensors. Preprints 2018, 2018030071.


The present Feature Paper highlights the application of bacterial surface (S-) layer proteins as versatile components for the fabrication of biosensors. One technologically relevant feature of S‑layer proteins is their ability to self-assemble on many surfaces and interfaces to form a crystalline 2D protein lattice. The S-layer lattice on the surface of a biosensor becomes part of the interface architecture, linking the bioreceptor to the transducer interface, which may cause signal amplification. The S-layer lattice as ultrathin, highly porous structure with functional groups in a well-defined special distribution and orientation and an overall anti-fouling characteristics can significantly raise the limit in terms of variety and ease of bioreceptor immobilization, compactness of bioreceptor molecule arrangement, sensitivity, specificity, and detection limit for many types of biosensors. The present paper discusses and summarizes examples for the successful implementation of S-layer lattices on biosensor surfaces in order to give a comprehensive overview on the application potential of these bioinspired S-layer protein-based biosensors.


biosensor; S-layer protein; crystalline 2D protein lattice; lipid membrane platform; linking matrix; bioreceptor; biomimetics


Biology and Life Sciences, Biophysics

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