Version 1
: Received: 29 January 2018 / Approved: 29 January 2018 / Online: 29 January 2018 (16:59:21 CET)
Version 2
: Received: 28 February 2018 / Approved: 1 March 2018 / Online: 1 March 2018 (14:24:15 CET)
How to cite:
Wambacq, E.; Audenaert, K.; Höfte, M.; De Saeger, S.; Haesaert, G. Bacillus Velezensis as Antagonist Towards Penicillium Roqueforti s.l. in Silage: In Vitro and In Vivo Evaluation. Preprints2018, 2018010273. https://doi.org/10.20944/preprints201801.0273.v2
Wambacq, E.; Audenaert, K.; Höfte, M.; De Saeger, S.; Haesaert, G. Bacillus Velezensis as Antagonist Towards Penicillium Roqueforti s.l. in Silage: In Vitro and In Vivo Evaluation. Preprints 2018, 2018010273. https://doi.org/10.20944/preprints201801.0273.v2
Wambacq, E.; Audenaert, K.; Höfte, M.; De Saeger, S.; Haesaert, G. Bacillus Velezensis as Antagonist Towards Penicillium Roqueforti s.l. in Silage: In Vitro and In Vivo Evaluation. Preprints2018, 2018010273. https://doi.org/10.20944/preprints201801.0273.v2
APA Style
Wambacq, E., Audenaert, K., Höfte, M., De Saeger, S., & Haesaert, G. (2018). <em>Bacillus Velezensis</em> as Antagonist Towards <em>Penicillium Roqueforti s.l.</em> in Silage: <em>In Vitro</em> and <em>In Vivo</em> Evaluation. Preprints. https://doi.org/10.20944/preprints201801.0273.v2
Chicago/Turabian Style
Wambacq, E., Sarah De Saeger and Geert Haesaert. 2018 "<em>Bacillus Velezensis</em> as Antagonist Towards <em>Penicillium Roqueforti s.l.</em> in Silage: <em>In Vitro</em> and <em>In Vivo</em> Evaluation" Preprints. https://doi.org/10.20944/preprints201801.0273.v2
Abstract
In Belgium, silages are often infected by Penicillium roqueforti sensu lato (s.l.). These toxigenic fungi are well adapted to silage conditions, and their prevention during feed-out is difficult. Bacillus velezensis strain NRRL B-23189 has been reported to inhibit P. roqueforti s.s. conidiospore germination in vitro by the production of lipopeptides. In the present study, the antagonistic effect of this B. velezensis strain towards P. roqueforti s.l. was evaluated in vitro and in vivo. In vitro, corn silage conditions were simulated, and the impact of B. velezensis culture supernatant or cell suspension on P. roqueforti s.l. growth, conidiospore germination and survival and roquefortine C production was evaluated. The antagonism was promising, but growth of B. velezensis in corn silage infusion was poor. An in vivo experiment with microsilos containing a mixture of perennial ryegrass and white clover artificially contaminated with P. roqueforti s.l. was carried out to determine if B. velezensis cell suspension could be used as an antagonistic silage inoculant. The B. velezensis cell suspension applied was unsuccessful in reducing P. roqueforti s.l. numbers at desiling after 56 days compared to no additive application. However, feed-out of the silage was not simulated, so it remains elusive whether or not B. velezensis exerts antagonistic activity during this phase.
Keywords
antagonism; Bacillus velezensis; Penicillium roqueforti; silage; roquefortine C
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
