Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective Detection

Antonino Foti
* ,
Cristiano D'andrea
ORCID logo ,
Valentina Villari
,
Norberto Micali
,
Maria Grazia Donato
,
Barbara Fazio
,
Onofrio Maria Maragò
,
Raymond Gillibert
ORCID logo ,
Marc Lamy de La Chapelle
,
Pietro Giuseppe Gucciardi
* ORCID logo
Version 1 : Received: 19 January 2018 / Approved: 19 January 2018 / Online: 19 January 2018 (09:34:08 CET)

A peer-reviewed article of this Preprint also exists.

Foti, A.; D’Andrea, C.; Villari, V.; Micali, N.; Donato, M.G.; Fazio, B.; Maragò, O.M.; Gillibert, R.; Lamy de la Chapelle, M.; Gucciardi, P.G. Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective Detection. Materials 2018, 11, 440. Foti, A.; D’Andrea, C.; Villari, V.; Micali, N.; Donato, M.G.; Fazio, B.; Maragò, O.M.; Gillibert, R.; Lamy de la Chapelle, M.; Gucciardi, P.G. Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective Detection. Materials 2018, 11, 440.

Abstract

Optical forces are used to aggregate plasmonic nanoparticles and create SERS-active hot spots in liquid. When biomolecules are added to the nanoparticles, high sensitivity SERS detection is accomplished. Here we tailor this methodology to detect catalase and hemoglobin, two Raman resonant biomolecules, at concentrations down to 10 nM and 1 pM. Subsequently, we study the SERS signal in Bovine Serum Albumin as a function of the concentration, finding a monotonic dependence that suggests the possibility of quantitative detection. Finally, by exploiting nanoparticles functionalized with specific aptamers, we obtain first results on the SERS detection of Ochratoxin A, a fungal toxin found in food commodities and wine. This represents a first step towards the addition of molecular specificity to this novel biosensor strategy.

Keywords

SERS; biosensor; gold nanoparticles; aptamers; toxins; hemeprotein; optical forces; optical tweezers; optical patterning; colloids.

Subject

Chemistry and Materials Science, Nanotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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