Preprint Article Version 1 This version not peer reviewed

Chemical Vapour Deposition Graphene Field-Effect Transistors for Detection of Human Chorionic Gonadotropin Cancer Risk Biomarker

Version 1 : Received: 14 December 2017 / Approved: 15 December 2017 / Online: 15 December 2017 (12:01:47 CET)
Version 2 : Received: 29 December 2017 / Approved: 2 January 2018 / Online: 2 January 2018 (05:21:45 CET)

How to cite: Haslam, C..; Damiati, S..; Whitley, T..; Davey, P..; Ifeachor, E..; Awan, S.A. Chemical Vapour Deposition Graphene Field-Effect Transistors for Detection of Human Chorionic Gonadotropin Cancer Risk Biomarker. Preprints 2017, 2017120106 (doi: 10.20944/preprints201712.0106.v1). Haslam, C..; Damiati, S..; Whitley, T..; Davey, P..; Ifeachor, E..; Awan, S.A. Chemical Vapour Deposition Graphene Field-Effect Transistors for Detection of Human Chorionic Gonadotropin Cancer Risk Biomarker. Preprints 2017, 2017120106 (doi: 10.20944/preprints201712.0106.v1).

Abstract

We report on the development of chemical vapour deposition (CVD) based graphene field effect transistor (GFET) immunosensors for the sensitive detection of Human Chorionic Gonadotropin (hCG), a glycoprotein risk biomarker of certain cancers. The GFET sensors were fabricated on Si/SiO2 substrate using photolithography with evaporated chromium and sputtered gold contacts. GFET channels were functionalized with a linker molecule to immobile anti-hCG antibody on the surface of graphene. Binding reaction of the antibody with varying concentration levels of hCG antigen demonstrated the limit of detection of the GFET sensors was ~0.1 pg/mL using four-probe electrical measurements. We also show annealing can significantly improve the carrier transport properties of GFETs and shift the Dirac point (Fermi level) with reduced p-doping in back-gated measurements. The developed GFET biosensors are generic and could find applications in a broad range of medical diagnostics in addition to cancer, such as neurodegenerative (Alzheimer’s, Parkinson’s and Lewy body) and cardiovascular disorders.

Subject Areas

graphene; electrochemical biosensors; cancer; diagnosis; electrical detection; alzheimer’s disease; dementia; neurodegenerative disorders; cardiovascular; blood biomarkers; antibodies; proteins

Readers' Comments and Ratings (0)

Leave a public comment
Send a private comment to the author(s)
Rate this article
Views 0
Downloads 0
Comments 0
Metrics 0
Leave a public comment

×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.