Preprint Article Version 1 This version is not peer-reviewed

Molecular and Biochemical Characterization of Calmodulin from Echinococcus granulosus

These authors contributed equally to this work.
Version 1 : Received: 23 May 2017 / Approved: 24 May 2017 / Online: 24 May 2017 (08:07:35 CEST)

How to cite: Wang, N.; Zhong, X.; Song, X.; Gu, X.; Lai, W.; Xie, Y.; Peng, X.; Yang, G. Molecular and Biochemical Characterization of Calmodulin from Echinococcus granulosus. Preprints 2017, 2017050173 (doi: 10.20944/preprints201705.0173.v1). Wang, N.; Zhong, X.; Song, X.; Gu, X.; Lai, W.; Xie, Y.; Peng, X.; Yang, G. Molecular and Biochemical Characterization of Calmodulin from Echinococcus granulosus. Preprints 2017, 2017050173 (doi: 10.20944/preprints201705.0173.v1).

Abstract

Echinococcus granulosus is a harmful cestode parasite which could cause Cystic Echinococcosis in humans, various livestock species and wild animals. Calmodulin (CaM), a Ca2+ sensor protein, is widely expressed in eukaryotes and mediates a variety of cellular signaling activities. In our study, the CaM in Echinococcus granulosus (rEgCaM) was successfully cloned and the molecular and biochemical characterizations of rEgCaM were identified. The results showed that rEgCaM was a highly conserved calcium-binding protein, consisting of 149 amino acids. Immunoblot analysis revealed that rEgCaM could be identified using E. granulosus infected sheep serum. The usage of rEgCaM as antigen was evaluated by indirect ELISA which exhibited a high sensitivity of 90.3% but low specificity (47.1%). rEgCaM was mainly located in the tegument tissues and parenchymal region of protoscoleces, the tegument and inner body of adult worm and predominantly expressed in the germinal layer. The mRNA expression of rEgCaM in PSCs were gradually decreased with the increase death of PSCs. In electrophoretic mobility tests and ANS assays, rEgCaM showed a typical calcium-binding protein characteristics. This is the first report on CaM from E. granulosus and rEgCaM is considered to be involved in some important biological function of E. granulosus as a calcium-binding protein.

Subject Areas

Echinococcus granulosus; Calmodulin; Ca2+-binding protein; Immunohistochemical localization; Quantitative RT-PCR

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