Engelhorn, J.; Blanvillain, R.; Kröner, C.; Parrinello, H.; Rohmer, M.; Posé, D.; Ott, F.; Schmid, M.; Carles, C.C. Dynamics of H3K4me3 Chromatin Marks Prevails over H3K27me3 for Gene Regulation during Flower Morphogenesis in Arabidopsisthaliana. Epigenomes2017, 1, 8.
Engelhorn, J.; Blanvillain, R.; Kröner, C.; Parrinello, H.; Rohmer, M.; Posé, D.; Ott, F.; Schmid, M.; Carles, C.C. Dynamics of H3K4me3 Chromatin Marks Prevails over H3K27me3 for Gene Regulation during Flower Morphogenesis in Arabidopsis thaliana. Epigenomes 2017, 1, 8.
Engelhorn, J.; Blanvillain, R.; Kröner, C.; Parrinello, H.; Rohmer, M.; Posé, D.; Ott, F.; Schmid, M.; Carles, C.C. Dynamics of H3K4me3 Chromatin Marks Prevails over H3K27me3 for Gene Regulation during Flower Morphogenesis in Arabidopsisthaliana. Epigenomes2017, 1, 8.
Engelhorn, J.; Blanvillain, R.; Kröner, C.; Parrinello, H.; Rohmer, M.; Posé, D.; Ott, F.; Schmid, M.; Carles, C.C. Dynamics of H3K4me3 Chromatin Marks Prevails over H3K27me3 for Gene Regulation during Flower Morphogenesis in Arabidopsis thaliana. Epigenomes 2017, 1, 8.
Abstract
Plant life-long organogenesis involves sequential, time and tissue specific expression of developmental genes. This requires activities of Polycomb Group (PcG) and trithorax Group complexes, respectively responsible for repressive Histone 3 trimethylation at lysine 27 (H3K27me3) and activation-related H3K4me3. However, the genome-wide dynamics in histone modifications that occur during developmental processes have remained elusive. Here, we report the distributions of H3K27me3 and H3K4me3 along with transcriptional changes, in a developmental series including Arabidopsis leaf and three stages of flower development. We found that chromatin mark levels are highly dynamic over the time series on nearly half of all Arabidopsis genes. Moreover, during early flower morphogenesis, changes in H3K4me3 prime over changes in H3K27me3 and quantitatively correlate with transcription changes, while H3K27me3 changes occur after prolonged expression changes. Notably, early activation of PcG target genes is dominated by increases in H3K4me3 while H3K27me3 remains present at the locus. Our results reveal H3K4me3 as greater predictor over H3K27me3 for transcription dynamics, unveil unexpected chromatin mechanisms at gene activation and underline the relevance of tissue-specific temporal epigenomics.
Keywords
Chromatin and transcription dynamics; reproductive development; differentiation; ChIP-seq; RNA-seq
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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