Preprint Article Version 1 This version not peer reviewed

Dynamics of H3K4me3 Chromatin Marks Take the Lead over H3K27me3 for Gene Regulation during Flower Morphogenesis in Arabidopsis thaliana

Version 1 : Received: 8 May 2017 / Approved: 8 May 2017 / Online: 8 May 2017 (18:25:10 CEST)

A peer-reviewed article of this Preprint also exists.

Engelhorn, J.; Blanvillain, R.; Kröner, C.; Parrinello, H.; Rohmer, M.; Posé, D.; Ott, F.; Schmid, M.; Carles, C.C. Dynamics of H3K4me3 Chromatin Marks Prevails over H3K27me3 for Gene Regulation during Flower Morphogenesis in Arabidopsis thaliana. Epigenomes 2017, 1, 8. Engelhorn, J.; Blanvillain, R.; Kröner, C.; Parrinello, H.; Rohmer, M.; Posé, D.; Ott, F.; Schmid, M.; Carles, C.C. Dynamics of H3K4me3 Chromatin Marks Prevails over H3K27me3 for Gene Regulation during Flower Morphogenesis in Arabidopsis thaliana. Epigenomes 2017, 1, 8.

Journal reference: Epigenomes 2017, 1, 8
DOI: 10.3390/epigenomes1020008

Abstract

Plant life-long organogenesis involves sequential, time and tissue specific expression of developmental genes. This requires activities of Polycomb Group (PcG) and trithorax Group complexes, respectively responsible for repressive Histone 3 trimethylation at lysine 27 (H3K27me3) and activation-related H3K4me3. However, the genome-wide dynamics in histone modifications that occur during developmental processes have remained elusive. Here, we report the distributions of H3K27me3 and H3K4me3 along with transcriptional changes, in a developmental series including Arabidopsis leaf and three stages of flower development. We found that chromatin mark levels are highly dynamic over the time series on nearly half of all Arabidopsis genes. Moreover, during early flower morphogenesis, changes in H3K4me3 prime over changes in H3K27me3 and quantitatively correlate with transcription changes, while H3K27me3 changes occur after prolonged expression changes. Notably, early activation of PcG target genes is dominated by increases in H3K4me3 while H3K27me3 remains present at the locus. Our results reveal H3K4me3 as greater predictor over H3K27me3 for transcription dynamics, unveil unexpected chromatin mechanisms at gene activation and underline the relevance of tissue-specific temporal epigenomics.

Subject Areas

Chromatin and transcription dynamics; reproductive development; differentiation; ChIP-seq; RNA-seq

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