Preprint Communication Version 1 This version is not peer-reviewed

Interconnection between Wheat ABCC13 Transporter and Auxin-Related Genes during Lateral Root Development

Version 1 : Received: 5 May 2017 / Approved: 8 May 2017 / Online: 8 May 2017 (07:48:20 CEST)

How to cite: Bhati, K.K.; Kumar, A.; Pandey, A.K. Interconnection between Wheat ABCC13 Transporter and Auxin-Related Genes during Lateral Root Development. Preprints 2017, 2017050050 (doi: 10.20944/preprints201705.0050.v1). Bhati, K.K.; Kumar, A.; Pandey, A.K. Interconnection between Wheat ABCC13 Transporter and Auxin-Related Genes during Lateral Root Development. Preprints 2017, 2017050050 (doi: 10.20944/preprints201705.0050.v1).

Abstract

TaABCC13 is member of wheat ABCC subclass of transporters. The RNAi mediated silencing of this transporter in wheat results in lowering of seed phytic acid level and other developmental defects. In addition to that, wheat ABCC13 was involved in cadmium detoxification as evident by the complementation assays in yeast. The appearance of early lateral roots in these transgenic seedlings speculated the possibility for studying the role of localized auxin-mediated effects. In the current study, firstly, the expression of auxin related genes was studied in the transgenic roots. Enhanced expression of genes pertaining to either auxin biosynthesis or its transport was observed in transgenic wheat seedling roots suggesting the direct effect of the hormone. Further, the early emergence of lateral roots in transgenic wheat seedlings was affected due to the presence of auxin-transport inhibitor suggesting the direct effect of hormones in root development. In conclusion, herein we provide the novel evidence for the auxin mediated regulation of lateral root emergence in TaABCC13:RNAi seedlings.

Subject Areas

ABCC transporter; lateral roots; Triticum aestivum; auxin; transport inhibition

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