Preprint Article Version 1 This version is not peer-reviewed

The Investigation of Electrochemistry Behaviors of Tyrosinase Based on Directly Electrodeposited Graphene on Choline–Gold Nanoparticals

Version 1 : Received: 27 April 2017 / Approved: 28 April 2017 / Online: 28 April 2017 (05:25:54 CEST)

A peer-reviewed article of this Preprint also exists.

He, Y.; Yang, X.; Han, Q.; Zheng, J. The Investigation of Electrochemistry Behaviors of Tyrosinase Based on Directly-Electrodeposited Grapheneon Choline-Gold Nanoparticles. Molecules 2017, 22, 1047. He, Y.; Yang, X.; Han, Q.; Zheng, J. The Investigation of Electrochemistry Behaviors of Tyrosinase Based on Directly-Electrodeposited Grapheneon Choline-Gold Nanoparticles. Molecules 2017, 22, 1047.

Journal reference: Molecules 2017, 22, 1047
DOI: 10.3390/molecules22071047

Abstract

A novel catechol (CA) biosensor was developed by the immobilization of tyrosinase (Tyr) onto in situ electrochemical reduction graphene (EGR) on choline functionalized gold nanoparticals (AuNPs–Ch) film. The results of UV–visible spectra indicated that Tyr retained its original structure in the film. And electrochemical investigation of the biosensor showed a pair of well–defined, quasi–reversible redox peaks with Epa= –0.0744V and Epc= –0.114 V (vs. SCE) in 0.1 M, pH 7.0 sodium phosphate buffered saline at the scan rate of 100 mV/s. The transfer rate constant ks was 0.66 s–1. The Tyr–EGR/AuNPs–Ch showed a good electrochemical catalytic response for the reduction of CA, with the linear range from 0.2 to 270 μM and a detection limit of 0.1 μM (S/N= 3). The apparent Michaelis–Menten constant was estimated to be 109 μM.

Subject Areas

electrochemistry; tyrosinase; graphene; Choline–gold nanoparticals; catechol

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