He, Y.; Yang, X.; Han, Q.; Zheng, J. The Investigation of Electrochemistry Behaviors of Tyrosinase Based on Directly-Electrodeposited Grapheneon Choline-Gold Nanoparticles. Molecules2017, 22, 1047.
He, Y.; Yang, X.; Han, Q.; Zheng, J. The Investigation of Electrochemistry Behaviors of Tyrosinase Based on Directly-Electrodeposited Grapheneon Choline-Gold Nanoparticles. Molecules 2017, 22, 1047.
He, Y.; Yang, X.; Han, Q.; Zheng, J. The Investigation of Electrochemistry Behaviors of Tyrosinase Based on Directly-Electrodeposited Grapheneon Choline-Gold Nanoparticles. Molecules2017, 22, 1047.
He, Y.; Yang, X.; Han, Q.; Zheng, J. The Investigation of Electrochemistry Behaviors of Tyrosinase Based on Directly-Electrodeposited Grapheneon Choline-Gold Nanoparticles. Molecules 2017, 22, 1047.
Abstract
A novel catechol (CA) biosensor was developed by the immobilization of tyrosinase (Tyr) onto in situ electrochemical reduction graphene (EGR) on choline functionalized gold nanoparticals (AuNPs–Ch) film. The results of UV–visible spectra indicated that Tyr retained its original structure in the film. And electrochemical investigation of the biosensor showed a pair of well–defined, quasi–reversible redox peaks with Epa= –0.0744V and Epc= –0.114 V (vs. SCE) in 0.1 M, pH 7.0 sodium phosphate buffered saline at the scan rate of 100 mV/s. The transfer rate constant ks was 0.66 s–1. The Tyr–EGR/AuNPs–Ch showed a good electrochemical catalytic response for the reduction of CA, with the linear range from 0.2 to 270 μM and a detection limit of 0.1 μM (S/N= 3). The apparent Michaelis–Menten constant was estimated to be 109 μM.
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.