Mor, F.; Sengul, O.; Topsakal, S.; Kilic, M.A.; Ozmen, O. Diabetogenic Effects of Ochratoxin A in Female Rats. Toxins2017, 9, 144.
Mor, F.; Sengul, O.; Topsakal, S.; Kilic, M.A.; Ozmen, O. Diabetogenic Effects of Ochratoxin A in Female Rats. Toxins 2017, 9, 144.
In this study, diabetogenic effects of long term Ochratoxin A (OTA) administration in rats were investigated and its role in the etiology of diabetes mellitus (DM) was examined utilizing 42 female Wistar rats for these purposes. The rats were divided into 3 different study and control groups according to the duration of the OTA administration. Rats received 45 μg OTA daily in their feed for 6, 9 and 24 weeks study groups. Three control groups without any treatment were also used in the same periods. Blood and pancreatic tissue samples were collected during the necropsy at the end of 6, 9 and 24 weeks. Plasma values of insulin, glucagon and glucose in study and control groups were determined. Pancreatic lesions were evaluated by histopathological examination; then insulin and glucagon expression in these lesions were determined by immunohistochemical methods. Statistically significant decrease in insulin levels in contrast to increases in glucagon and glucose levels in blood were observed. Slight degeneration in Langerhans islet cells were observed at the histopathological examination in all OTA treated groups. Immunohistochemistry of pancreatic tissue revealed decreased insulin and increased glucagon expression. This study demonstrated that OTA may cause pancreatic damage in Langerhans islet and predispose rats to DM.
Ochratoxin A, insulin, glucagon, glucose, rat plasma, pathology, immunohistochemistry
MEDICINE & PHARMACOLOGY, Pathology & Pathobiology
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