Preprint Short Note Version 1 This version not peer reviewed

Chemosyndrome Variation in Mycobiont Culture and Thallus of Parmelina carporrhizans and Parmelina quercina

Version 1 : Received: 30 March 2017 / Approved: 30 March 2017 / Online: 30 March 2017 (16:58:33 CEST)

How to cite: Alors, D.; Divakar, P.K.; Meiser, A.; Schmitt, I.; Crespo, A.; Molina, M.C. Chemosyndrome Variation in Mycobiont Culture and Thallus of Parmelina carporrhizans and Parmelina quercina. Preprints 2017, 2017030219 (doi: 10.20944/preprints201703.0219.v1). Alors, D.; Divakar, P.K.; Meiser, A.; Schmitt, I.; Crespo, A.; Molina, M.C. Chemosyndrome Variation in Mycobiont Culture and Thallus of Parmelina carporrhizans and Parmelina quercina. Preprints 2017, 2017030219 (doi: 10.20944/preprints201703.0219.v1).

Abstract

We cultured Parmelina carporrhizans and P. quercina in Corn Meal Agar and 0.2% glucose Malt Yeast Agar for 160 days. Chemosyndrome of natural thalli and mycobiont cultures were analyzed by HPLC. Lecanoric acid, atranorin, chloratranorin and ergosterol were detected in P. carporrhizans thalli, while lecanoric acid, chloratranorin and aliphates were found in P. quercina thalli. The secondary methabolites pattern between thalli and mycobiont culture was completely different in both species. Both species secreted the phenalenone myeloconone C in culture media and was also detected in P. quercina mycobiont aggregates. Interestingly, the phenolic compounds produced by the mycobiont culture of P. carporrhizans are related to those produced by natural thallus by the same biosynthetic pathway, while the chemosyndrome of P. quercina mycobiont implies switch of biosynthetic pathway from acetate-polymalonate pathway to shikimic acid pathway, with pulvinic acid as major compound of mycobiont culture. The role of Myelochonone C, confluentic acid and pulvinic acid produced by mycobiont culture is discussed as possible adaptive vantage in field as photoprotective agent or as byproduct result of stressing artificial culture conditions.

Subject Areas

Parmelina; phenols; HPLC; myeloconone C

Readers' Comments and Ratings (1)

Comment 1
Received: 17 May 2017
Commenter: David Alors
The commenter has declared there is no conflict of interests.
Comment: We are under review of this paper, and some corrections are done, Trying to confirm the presence of Myeloconone C, we didnt find it, actually is discard. The secreted substance is unknown for us.

Thanks to all the people reading our work.

David
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