Version 1
: Received: 15 March 2017 / Approved: 16 March 2017 / Online: 16 March 2017 (09:35:36 CET)
How to cite:
Riyaphan, J.; Jhong, C.; Tsai, M.; Lee, D.; Leong, M.K.; Weng, C. Potent Natural Inhibitors of Alpha-Glucosidase and Alpha-Amylase against Hyperglycemia in Vitro and in Vivo. Preprints2017, 2017030116. https://doi.org/10.20944/preprints201703.0116.v1
Riyaphan, J.; Jhong, C.; Tsai, M.; Lee, D.; Leong, M.K.; Weng, C. Potent Natural Inhibitors of Alpha-Glucosidase and Alpha-Amylase against Hyperglycemia in Vitro and in Vivo. Preprints 2017, 2017030116. https://doi.org/10.20944/preprints201703.0116.v1
Riyaphan, J.; Jhong, C.; Tsai, M.; Lee, D.; Leong, M.K.; Weng, C. Potent Natural Inhibitors of Alpha-Glucosidase and Alpha-Amylase against Hyperglycemia in Vitro and in Vivo. Preprints2017, 2017030116. https://doi.org/10.20944/preprints201703.0116.v1
APA Style
Riyaphan, J., Jhong, C., Tsai, M., Lee, D., Leong, M.K., & Weng, C. (2017). Potent Natural Inhibitors of Alpha-Glucosidase and Alpha-Amylase against Hyperglycemia <em>in Vitro </em>and <em>in Vivo</em>. Preprints. https://doi.org/10.20944/preprints201703.0116.v1
Chicago/Turabian Style
Riyaphan, J., Max K. Leong and Ching-Feng Weng. 2017 "Potent Natural Inhibitors of Alpha-Glucosidase and Alpha-Amylase against Hyperglycemia <em>in Vitro </em>and <em>in Vivo</em>" Preprints. https://doi.org/10.20944/preprints201703.0116.v1
Abstract
The inhibition of alpha-glucosidase and alpha-amylase is one of clinic strategies for remedy the type II diabetes. Herbal medicines are reported to alleviate hyperglycemia. However, the constituents from those sources whether are targeted to the alpha-glucosidase and alpha-amylase still unexplored. This study attempted to select the compounds for efficacy of hypoglycemia via cellular and mouse levels. The results illustrated that the cytotoxicity in all tested compounds at various concentrations except the concentration of 16-hydroxy-cleroda-3,13-dine-16,15-olide (HCD) at 30 µM were not significant difference (p > 0.05) when compared with the untreated control. Acarbose (reference drug), Antroquinonol, Catechin, Quercetin, Actinodaphnine, Curcumin, HCD, Docosanol, Tetracosanol, Berberine, and Rutin could effectively inhibit the alpha-glucosidase activity of Caco-2 cells when compared with the control (maltose). The compounds (Curcumin, HCD, Tetracosanol, Antroquinonol, Berberine, Catechin, Actinodaphnine, and Rutin) could reduce blood sugar level at 30 min in tested mice. The effects of tested compounds on area under curve (AUC) were significant (p < 0.05) among Acarbose, Tetracosanol, Antroquinonol, Catechin, Actinodaphnine, and Rutin along with Berberine and Quercetin. In in vitro (alpha-glucosidase) with in vivo (alpha-amylase) experiments suggest that bioactive compounds can be a potential inhibitor candidate of alpha-glucosidase and alpha-amylase for the alleviation of type II diabetes.
Keywords
In vivo; Alpha-glucosidase; Alpha-amylase; hyperglycemia
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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