Version 1
: Received: 14 March 2017 / Approved: 14 March 2017 / Online: 14 March 2017 (13:43:13 CET)
How to cite:
Rabadan-Ros, R.; Aznar-Cervantes, S.; Mazón, P.; Ros-Tarraga, P.; DE AZA, P.N.; MESEGUER-OLMO, L. Nurse´s A-phase material enhance adhesion, growth and differentiation of human bone marrow-derived stromal mesenchymal stem cells. Preprints2017, 2017030085. https://doi.org/10.20944/preprints201703.0085.v1
Rabadan-Ros, R.; Aznar-Cervantes, S.; Mazón, P.; Ros-Tarraga, P.; DE AZA, P.N.; MESEGUER-OLMO, L. Nurse´s A-phase material enhance adhesion, growth and differentiation of human bone marrow-derived stromal mesenchymal stem cells. Preprints 2017, 2017030085. https://doi.org/10.20944/preprints201703.0085.v1
Rabadan-Ros, R.; Aznar-Cervantes, S.; Mazón, P.; Ros-Tarraga, P.; DE AZA, P.N.; MESEGUER-OLMO, L. Nurse´s A-phase material enhance adhesion, growth and differentiation of human bone marrow-derived stromal mesenchymal stem cells. Preprints2017, 2017030085. https://doi.org/10.20944/preprints201703.0085.v1
APA Style
Rabadan-Ros, R., Aznar-Cervantes, S., Mazón, P., Ros-Tarraga, P., DE AZA, P.N., & MESEGUER-OLMO, L. (2017). Nurse´s A-phase material enhance adhesion, growth and differentiation of human bone marrow-derived stromal mesenchymal stem cells. Preprints. https://doi.org/10.20944/preprints201703.0085.v1
Chicago/Turabian Style
Rabadan-Ros, R., PIEDAD N. DE AZA and LUIS MESEGUER-OLMO. 2017 "Nurse´s A-phase material enhance adhesion, growth and differentiation of human bone marrow-derived stromal mesenchymal stem cells" Preprints. https://doi.org/10.20944/preprints201703.0085.v1
Abstract
The purpose of this study was to evaluate the bioactivity and cell response of a well-characterized Nurse´s A-phase (7CaO•P2O5•2SiO2) ceramic and his effect compared to a control (tissue culture polystyrene-TCPS) on the adhesion, viability, proliferation and osteogenic differentiation of ahMSCs in vitro. Cell proliferation (Alamar Blue Assay), Alizarin Red-S (AR-s) staining, alkaline phosphatase (ALP) activity, osteocalcin (OCN) and collagen I (Col I) were evaluated. Also, field emission scanning electron microscopy (FESEM) images were acquired in order to visualise the cells and the topography of the material. The proliferation of cells growing in a direct contact with the material was slower at early stages of the study because of the new environmental conditions. However, the entire surface was colonized after 28 days of culture in growth medium (GM). Osteoblastic differentiation markers were significantly enhanced in cells growing on Nurse´s A phase ceramic and cultured with osteogenic medium (OM), probably due to the role of silica to stimulate the differentiation of ahMSCs. Moreover, calcium nodules were formed under the influence of ceramic material. Therefore, it is predicted that Nurse´s A-phase ceramic would present high biocompatibility and osteoinductive properties being a good candidate to be used as a biomaterial for bone tissue engineering.
Keywords
TCP-C2S, Nurse ´A ceramic, Biomaterials, adult human mesenchymal stem cells, Solid State Reaction, Biomedical applications.
Subject
Chemistry and Materials Science, Biomaterials
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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