Preprint Article Version 1 This version is not peer-reviewed

Detection of 17 β-estradiol in Environmental Estrogen Pollution and in Women’s Health Care Using a Single-Use, Cost Effective Biosensor Based on Differential Pulse Voltammetry (DPV)

Version 1 : Received: 7 March 2017 / Approved: 8 March 2017 / Online: 8 March 2017 (05:39:59 CET)

A peer-reviewed article of this Preprint also exists.

Dai, Y.; Liu, C.C. Detection of 17 β-Estradiol in Environmental Samples and for Health Care Using a Single-Use, Cost-Effective Biosensor Based on Differential Pulse Voltammetry (DPV). Biosensors 2017, 7, 15. Dai, Y.; Liu, C.C. Detection of 17 β-Estradiol in Environmental Samples and for Health Care Using a Single-Use, Cost-Effective Biosensor Based on Differential Pulse Voltammetry (DPV). Biosensors 2017, 7, 15.

Journal reference: Biosensors 2017, 7, 15
DOI: 10.3390/bios7020015

Abstract

Environmental estrogen pollution and estrogen effects on the female reproduction system are well-recognized scientifically. Among the estrogens, 17 β-estradiol is a priority in environmental estrogen pollution, and it is also a major contributor to estrogen which regulates the female reproduction system. 17 β-estradiol is carcinogen and has a tumor promotion effect relating to breast cancer, lung cancer and others. It also affects the psychological well-being such as depression, fatigue and others. Thus, a simple method of detection of 17 β-estradiol will be important for both environmental estrogen pollution and women’s health care. This study demonstrates a simple-use, cost effective 17 β-estradiol biosensor system which can be used for both environment and women’s health care applications. The bio-recognition mechanism is based on the influence of the redox couple, K3Fe (CN) 6/K4Fe (CN) 6 by the interaction between 17 β-estradiol antigen and its α-receptor (ER- α; α-estrogen antibody). The transduction mechanism is an electrochemical analytical technique, differential pulse voltammetry (DPV). The levels of 17 β-estradiol antigen studied was between 2.25 pg/mL to 2,250 pg/mL, Phosphate buffered saline (PBS), tap water from the Cleveland regional water district, and simulate urine were used as the test media covering the potential application areas for 17 β-estradiol detection. An interference study by testosterone which has a similar chemical structure and molecular weight as those of 17 β-estradiol was carried out, and this 17 β-estradiol biosensor showed excellent specificity without any interference by similar chemicals.

Subject Areas

17 β-estradiol; estrogen pollution; female reproduction; DPV

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