Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

In Vivo Imaging of Local Gene Expression Induced by Magnetic Hyperthermia

Olivier Sandre
* ORCID logo ,
Coralie Genevois
,
Eneko Garaio
,
Laurent Adumeau
,
Stéphane Mornet
ORCID logo ,
Franck Couillaud
* ORCID logo
Version 1 : Received: 23 January 2017 / Approved: 24 January 2017 / Online: 24 January 2017 (04:14:19 CET)

A peer-reviewed article of this Preprint also exists.

Sandre, O.; Genevois, C.; Garaio, E.; Adumeau, L.; Mornet, S.; Couillaud, F. In Vivo Imaging of Local Gene Expression Induced by Magnetic Hyperthermia. Genes 2017, 8, 61. Sandre, O.; Genevois, C.; Garaio, E.; Adumeau, L.; Mornet, S.; Couillaud, F. In Vivo Imaging of Local Gene Expression Induced by Magnetic Hyperthermia. Genes 2017, 8, 61.

Abstract

The present work aims to demonstrate that colloidal dispersions of magnetic iron oxide nanoparticles stabilized with dextran macromolecules placed in an alternating magnetic field can not only produce heat, but also that these particles could be used in vivo for local and non-invasive deposition of a thermal dose sufficient to trigger thermo-induced gene expression. Iron oxide nanoparticles were first characterized in vitro on a bio-inspired setup, and then they were assayed in vivo using a transgenic mouse strain expressing the luciferase reporter gene under transcriptional control of a thermosensitive promoter. Iron oxide nanoparticles dispersions were applied topically on the mouse skin or injected sub-cutaneously with Matrigel™ to generate so called pseudo tumors. Temperature was monitored continuously with a feedback loop to control the power of the magnetic field generator and to avoid overheating. Thermo-induced luciferase expression was followed by bioluminescence imaging 6 hours after heating. We showed that dextran-coated magnetic iron oxide nanoparticles dispersions were able to induce in vivo mild hyperthermia compatible with thermo-induced gene expression in surrounding tissues and without impairing cell viability. These data open new therapeutic perspectives for using mild magnetic hyperthermia as non-invasive modulation of tumor microenvironment by local thermo-induced gene expression or drug release.

Keywords

magnetic hyperthermia; gene therapies; heat shock protein promoter; in vivo optical imaging; magnetic polymer-coated nanoparticles

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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