Preprint Article Version 1 NOT YET PEER-REVIEWED

Synthesis of Reusable Silica Nanosphere-Supported Pt(IV) Complex for Formation of Disulfide Bonds in Peptides

Version 1 : Received: 12 December 2016 / Approved: 12 December 2016 / Online: 12 December 2016 (17:54:08 CET)

A peer-reviewed article of this Preprint also exists.

Hou, X.; Zhao, X.; Zhang, Y.; Han, A.; Huo, S.; Shen, S. Synthesis of Reusable Silica Nanosphere-Supported Pt(IV) Complex for Formation of Disulfide Bonds in Peptides. Molecules 2017, 22, 338. Hou, X.; Zhao, X.; Zhang, Y.; Han, A.; Huo, S.; Shen, S. Synthesis of Reusable Silica Nanosphere-Supported Pt(IV) Complex for Formation of Disulfide Bonds in Peptides. Molecules 2017, 22, 338.

Journal reference: Molecules 2017, 22, 338
DOI: 10.3390/molecules22020338

Abstract

Some peptide-based drugs including oxytocin, vasopressin, ziconotide, pramlintide, nesiritide, and octreotide, contain one intramolecular disulfide bonds. A novel and reusable monodispersed silica nanosphere-supported Pt(IV) complex (SiO2@TPEA@Pt(IV)) was synthesized via a four-step procedure and used for formation of intramolecular disulfide bonds in peptides. TEM and chemical mapping results for the Pt(II) intermediates and for SiO2@TPEA@Pt(IV) show that the silica nanospheres possess a monodisperse spherical structure and contain uniformly distributed Si, O, C, N, Cl, and Pt. The valence state of Pt on the silica nanospheres was characterized by XPS. The Pt(IV) loaded on SiO2@TPEA@Pt(IV) was 0.15 mmol/g, as determined by UV-vis spectrometry. Formation of intramolecular disulfides in six dithiol-containing peptides of variable lengths by use of SiO2@TPEA@Pt(IV) was investigated and the relative oxidation yields were determined by HPLC. In addition, peptide 1 (Ac-CPFC-NH2) was utilized to study the reusability of SiO2@TPEA@Pt(IV). No significant decrease in the relative oxidation yield was observed after ten reaction cycles. Moreover, the structure of SiO2@TPEA@Pt(IV) after being used ten cycles was determined to be similar to its initial one, demonstrating the cycling stability of the complex.

Subject Areas

monodisperse silica nanospheres; supported platinum(IV) complex; peptide; intramolecular disulfide; reusability

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