Giant unilamellar vesicles (GUVs) are membrane model used to study membrane properties. Electroformation is one of the methods used to produce GUVs. During electroformation protocol dry lipid film is formed. Drying of the lipid film induce Cholesterol (Chol) demixing artifact, in which Chol forms anhydrous crystals, where these crystals does not participate in formation of vesicles. This leads to a lower Chol concentration in the vesicle bilayers compared to the Chol concentration in the initial lipid solution. To address this problem, we propose novel electroformation protocol that includes rapid solvent exchange, plasma cleaning and spin-coating methods to produce GUVs. We tested the protocol focusing on vesicles with high Chol content using different spin-coating durations and vesicle types deposition. Additionally, we compared the novel protocol using completely dry lipid film. The optimal spin-coating duration for vesicles made out of phosphatidylcholine/Chol mixture was 30 s. Mulitlamellar vesicles (MLVs), large unilamellar vesicles (LUVs) obtained by extrusion of MLVs through 100 nm membrane pores and LUVs obtained by extrusion of previously obtained LUVs through 50 nm membrane pores were deposited on electrode for 1.5/1 Chol/phosphatidylcholine lipid mixture and the results were compared. Electroformation using all three deposited vesicle types gave a high GUVs yield, but deposition of LUVs obtained by extrusion of MLVs through 100 nm membrane pores gave the most reproducible results. Using deposition of these LUVs, we produced GUVs with high yield for 6 different Chol concentrations (from 0 % to 71.4%). Using protocol that includes dry lipid film GUVs yield was lower and Chol demixing artifact was induced, proving that the lipid film should never undergo drying when Chol content is high.

Rising humidity in old and historical buildings is a current issue that affects their structure, due to water capillarity and its propagation inside the walls, a key factor in heritage preserving and its functionality. It is also responsible for materials degradation exposed to frost and defrost cycles, biodegradation and salt crystallization. Moisture also increases the heating costs of the affected buildings, due to high thermal conductivity of the moist masonries. The paper presents an unconventional method for humidity removal or stabilisation due to infiltration of capillary water into the masonries, using electromagnetic waves systems that result in changing the water polarity inside the affected walls and pushing it out of the construction. The system consist of a small box, connected to the power supply, installed inside the building, remaining on the same location throughout the entire life of the construction. By means of this procedure, not only do the walls of the structure stay moisture-free, but also the foundations and the surrounding soil. Through this process, not only the walls of the construction remains dry, but also the foundations and the soil around them. The electromagnetic wave method was applied on five different historical buildings, located in Romania, Cluj Napoca’s historical centre, in the vicinity of Somes river, with rising damp due to water capillarity. Measurements were conducted at every six months after the system installation, for a period of minimum 24 months, using the same marked locations, at a depth of 5 cm in the walls. All buildings studied are made of stone and stone with plain brick, with different chemical, type, pore direction and porosity characteristics. The measured values show in most cases, a clear decrease of wall humidity levels or a stagnation of the capillarity rise in the monitored walls, after a minimum of 24 months after the system installation. The electromagnetic wave system, as a non-destructive method, can be applied to cases with no accidental water source or high raised groundwater, for historical buildings where no exterior works are possible or interior invasive interventions.