Grapevine Trunk Diseases (GTDs) are caused by a wide variety of phytopathogenic fungal species that compromise grapevine productivity and wine quality. Each year they lead to major economic losses. Most GTD treatments currently available on the market are chemical-based and environ-mentally harmful. Given that one of the goals proposed by the European Green Deal is to develop sustainable agriculture which does not harm the environment, reduces pesticide use, and pro-motes rural area economies, an alternative to those treatments may be the use of elicitors such as oligosaccharides from fungal organisms. Indeed, many studies have confirmed that oligosaccha-rides play important roles in activating plants' innate immunity. Mannans are one possible type of such oligosaccharides. The present work analysed Vitis vinifera L. (cv. Tempranillo and cv. Airén) leaves and grapes to evaluate their physiological and molecular response to GTDs and how this response might be conditioned using different concentrations of mannans as elicitors. It was found that mannan addition to diseased vines was more positive for the leaves than for the grapes. In general, mannans increased the expression levels of chalcone synthase (CHS1, CHS3), phenylalanine ammonia lyase (PAL), superoxide dismutase (SOD), and polyphenol oxidase (PPO) in healthy leaves of both varieties. In diseased leaves, CHS3 and PAL decreased in both varieties, while CHS1 and PPO increased in Tempranillo but not in Airén. In the grapes, the expression pat-terns of the genes studied varied greatly due to the development of the disease. The mannan treatment seemed to reduce the oxidative stress caused by GTDs, although this response depend-ed on the variety and on the plant's phytosanitary status.

The possibility of applying Mo and Cr coatings by CVD method to the inner surfaces of tubular products Ø ˂ 60 mm has been studied.The process of applying Mo and Cr coatings by the method of gas-phase deposition was developed.The areas of parameters for obtaining coatings with a uniform structure, speed, hardness, as well as patterns of change in these characteristics when changing the main parameters of the process of obtaining such coatings are determined.The proven technological processes of applying Mo and Cr CVD coatings can be the basis for the development of pilot technologies for applying functional coatings to precision parts with small-diameter inner tubular surfaces in the mechanical engineering and aviation industries.A controlled process of applying two-component chromium-aluminum coatings by gas-phase deposition using organic and organometallic substances was developed. Optimization of the processes of applying high-quality two-component chromium-aluminum coatings on prototype models of the internal cavities of cooled turbine blades was carried out.The studies revealed the characteristics of two-component chromium-aluminum coatings comparable to those of coatings used in production, while ensuring good quality coatings in terms of adhesion strength to the turbine blade material. The reproducibility of the coatings obtained makes it possible to develop pilot technologies for production in the future.The lower toxicity of the precursors used makes it possible to create an environmentally friendly technology and significantly reduce environmental problems in production when applying two-component chromium-aluminum coatings to the internal cavities of cooled turbine blades.

In vineyards, Botryosphaeria dieback (BD) occurs in young and old plants. In the field, the prevalence and severity of the disease increase proportionally with the age of the vineyards. Among the pathogens that cause (BD), Diplodia seriata is the most prevalent species in Chile and other countries with a Mediterranean climate. To date, no information is available on the sus-ceptibility of adult wood to infection by this pathogen since most of the pathogenicity tests have been carried out on 1- or 2-year-old shoots or detached canes. Therefore, a pathogenicity test was carried out on plants under field conditions, with inoculations in plants, on one-year-old shoots, and 2- and 10-year-old wood in grapevine cv. Cabernet Sauvignon. A pathogenicity test was carried out with two isolates of D. seriata selected in an assay in detached canes that were 2-year-old. The results on plants showed that D. seriata was significantly more aggressive on 10-year-old than on one- or two-year-old tissue. These results were compared with the lesions ob-tained from two-year-old canes. The results of this work are consistent with the damage ob-served in the field, highlight the importance of identifying each pathogenic species that causes (BD) and contribute to the knowledge of the epidemiology of this disease in Mediterranean cli-mates.