The use of renewable fuels for internal combustion engines marks a significant stride towards sustainability in transportation and power generation. Ensuring the compatibility of these fuels with existing diesel engines and infrastructure is paramount for a smooth transition. Diesel engines ca-pable of harnessing the advantages of alternative fuels without extensive modifications offer a pragmatic approach to sustainable power for vehicles and industries. This article investigates the impact of carbon neutral renewable fuel types (HVOs and biodiesel) on the operation of the high-pressure injection pump, focusing on rotational speed and delivery pressure as key parame-ters. Investigations, based on pump operating cycle analysis and volumetric efficiency measure-ments, reveals that while speed dependence is moderate, fuel type significantly influences pump performance. Hydrogenated carbon-neutral fluids (HVOs) demonstrate adequate capabilities compared to conventional fossil fuels, offering promising prospects for reducing environmental impact. The findings underscore the importance of considering fuel properties, particularly com-pressibility modulus. These insights provide valuable guidance for the advancement of sustainable transportation and industrial systems.