Complementary Interaction of BmiRNA with mRNA of Human Transcription Factor Genes

Interest in the properties of miRNAs has significantly declined in recent years, largely due to inadequate conceptualizations of their interactions with mRNA. Recent publications have shown a growing tendency to consider fully complementary miRNA interactions with mRNA of transcription factor genes. In the present study, we examined the miRNAs identified by Backes et al. which we have designated as BmiRNA to distinguish them from those in the NCBI database and other standard miRNAs. The MirTarget software was utilized to determine the quantitative physicochemical characteristics of the interactions between BmiRNAs and target mRNAs. BmiRNAs differ significantly from other miRNAs across numerous properties. We observed that BmiRNAs bind with full complementarity to the mRNAs of numerous transcription factor genes. Specifically, it is demonstrated that the mRNAs of ZNF genes and other transcription factors bind to BmiRNAs with full complementarity. Nearly all these BmiRNAs exhibited binding within the 3′UTR region. We identified BmiRNAs that interact with multiple target genes, as well as target genes that interact with multiple BmiRNAs, and defined groups of BmiRNAs that bind to the mRNAs of multiple genes. Furthermore, we identified BmiRNAs interacting with the mRNAs of transcription factor genes with high free energy. These results, regarding the interaction of BmiRNAs with the mRNAs of a significant number of genes, support the hypothesis that miRNAs serve as critical regulators of human genome expression.