Acoustic Vibration as Mechanical Stimulation Modulates Actin Organization in Yeast

Human language continuously generates patterned mechanical vibrations, yet the cellular consequences of such structured sound remain largely unexplored. Here, we investigate whether acoustic vibration with different degrees of temporal and spectral organization modulates actin cable architecture and polarity in the non auditory eukaryote Saccharomyces cerevisiae. Using a direct contact stimulation setup, yeast cells expressing ABP140- GFP were exposed to sustained tonal sound, broadband white noise, or brief consonant like acoustic bursts designed to isolate speech relevant temporal structures without semantic content. Sustained tonal stimulation, characterized by rhythmic continuity and harmonic coherence similar to vowel like components of speech, increased ABP140- GFP signal intensity,actin branching and actin length and significantly enhanced shmoo formation. In contrast, broadband noise disrupted actin organization and suppressed shmooing, while transient consonant like bursts produced no measurable structural effects. These results indicate that language related acoustic structure, specifically sustained and coherent mechanical vibration, can modulate cytoskeletal organization in yeast, supporting the view of sound and speech as biologically active mechanical inputs rather than purely communicative signals.