This study examines women’s textile knowledge in Portugal as a fundamental element of cultural heritage, situating it within domestic, social, and industrial contexts, with a particular focus on Guimarães. Drawing on a multidisciplinary approach grounded in historical and documentary evidence, it analyses how female expertise in spinning, weaving, embroidery, and lacemaking contributed to the evolution of textile practices from the fifteenth century to the present day. The findings indicate that this knowledge was pivotal to the transformation of domestic textile activities into an emerging industrial sector, shaping both production methods and cultural identity. The study concludes that recognising the historical importance of women’s textile labour is essential for understanding the development of the Portuguese industry. Furthermore, this research emphasises the urgency of preserving, transmitting, and legitimising the intangible cultural heritage inextricably linked to women’s textile mastery. It argues that integrating this legacy into contemporary creative and industrial practices can foster cultural sustainability and unlock new possibilities for future innovation, ensuring that this ancestral expertise remains a living pillar of regional identity.